Probe:	Probe B
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent		4A

Structure	Level	Pattern	Image	Note
TS14: embryo	Absent		4B

Structure	Level	Pattern	Image	Note
TS15: embryo	Weak	Diffuse	4C
TS15: future brain	Present		4C
TS15: future spinal cord	Present		4C
TS15: otocyst	Present		4C

Structure	Level	Pattern	Image	Note
TS16: embryo	Weak	Diffuse	4D
TS16: future hindbrain	Present		4D
TS16: future midbrain	Present		4D
TS16: telencephalon	Present		4D

Structure	Level	Pattern	Image	Note
TS17: embryo	Weak	Diffuse	4E
TS17: hindbrain	Present		4E
TS17: midbrain	Present		4E
TS17: telencephalon	Present		4E

Structure	Level	Pattern	Image	Note
TS16: future spinal cord	Present		4F
TS16: future hindbrain	Present	Regionally restricted	4F	Expression was detected in the rhombic lip.

Structure	Level	Pattern	Image	Note
TS16: future spinal cord	Present		4G
TS16: telencephalon	Present		4G

Structure	Level	Pattern	Image	Note
TS17: midbrain	Strong		4H
TS17: telencephalon	Strong		4H

Structure	Level	Pattern	Image	Note
TS20: brain	Strong	Regionally restricted	4J	Most regions of the brain have strong expression within and outside the ventricular zone.
TS20: brain ventricular layer	Strong		4J

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Strong	Regionally restricted	4K	Strong expression was observed in the spinal cord both within and outside of the ventricular zone.
TS17: dorsal root ganglion	Present		4K

Structure	Level	Pattern	Image	Note
TS17: brain ventricular layer	Strong		4L
TS17: hindbrain	Strong		4L
TS17: midbrain	Strong		4L
TS17: telencephalon	Strong		4L

Structure	Level	Pattern	Image	Note
TS27: brain	Weak	Widespread	5A
TS27: main olfactory bulb mitral cell layer	Present		5A , 5C
TS27: main olfactory bulb granule cell layer	Present	Regionally restricted	5A , 5C	Expression was in the internal granular layer.
TS27: main olfactory bulb external plexiform layer	Absent		5C , 5A
TS27: main olfactory bulb glomerular layer	Absent		5C , 5A
TS27: cerebral cortex	Present		5A
TS27: cerebellum granule cell layer	Present		5A , 5E

Structure	Level	Pattern	Image	Note
TS27: cortical layer II	Present		5B
TS27: cortical layer III	Present		5B
TS27: cortical layer IV	Present		5B
TS27: cortical layer V	Present		5B
TS27: cortical layer VI	Present		5B
TS27: dentate gyrus granule cell layer	Present		5B , 5D
TS27: hippocampus CA3	Present		5B , 5D
TS27: thalamus	Present	Scattered	5B , 5F	Scattered punctate expression was detected. No expression was detected in the habenulopeduncular tracts.
TS27: hypothalamus	Present	Scattered	5B	Scattered punctate expression was detected.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Diffuse	6A , 6B	Expression was dense.
TS28: piriform cortex	Present		6B , 6A	Expression was dense.
TS28: cerebral hemisphere white matter	Absent		6B , 6A

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong	Diffuse	6D , 6C	Heavy, diffuse expression throughout the cortex.
TS28: piriform cortex	Strong		6D , 6C
TS28: hippocampus CA1	Strong		6D , 6C
TS28: dentate gyrus granule cell layer	Strong		6C
TS28: hippocampus CA3	Strong		6C
TS28: thalamus	Trace	Scattered	6C	Very little to no expression was in the thalamus.
TS28: cerebral hemisphere white matter	Absent		6D , 6C

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		6F , 6E
TS28: cerebellum granule cell layer	Weak	Diffuse	6E , 6F
TS28: cerebellum molecular layer	Weak	Diffuse	6E , 6F
TS28: cerebellum white matter	Absent		6E , 6F
TS28: brainstem	Present	Punctate	6E	Punctate hybridization was detected in the medullary brain stem.
TS28: medulla oblongata	Present	Scattered	6E

Structure	Level	Pattern	Image	Note
TS28: corpus callosum	Absent
TS28: hypothalamus	Trace	Scattered