Summary | Mutation origin | Mutation description | Recombinase activity | Phenotypes | Find Mice (IMSR) | References

Symbol:	Snap25tm2.1(cre)Hze
Name:	synaptosomal-associated protein 25; targeted mutation 2.1, Hongkui Zeng
MGI ID:	MGI:5507846
Synonyms:	Snap25-IRES2-Cre, Snap25-IRES2-Cre-D, Snap25-IRES2-Cre-Delta, Snap25-IRES2-Cre-DeltaNeo/Hygro, Snap25m1(IRES2-Cre)Hze
Gene:	Snap25  Location: Chr2:136555373-136624348 bp, + strand  Genetic Position: Chr2, 67.56 cM
Alliance:	Snap25tm2.1(cre)Hze page

Germline Transmission:	Earliest citation of germline transmission: J:146821
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Allele Type:    Targeted (Recombinase) Mutation:    Insertion   Snap25tm2.1(cre)Hze expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Snap25 (MGI:98331)   Mutation details: The targeting vector contained, from 5' to 3', a partial Snap25 sequence spanning intron 6 through intron 7, an frt3 site within Snap25 intron 7, a partial Snap25 exon 8 sequence up to and including the endogenous stop codon, an internal ribosome entry site 2 (IRES2) sequence, a cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 late polyA signal, and an AttP site. This construct was electroporated into G4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric mice were bred to PhiC31-expressing mice to remove the AttB/AttP-flanked sequences (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replace it with the recombined AttB/AttP site (AttL). The resulting Snap25-IRES2-Cre-D mice were bred with C57BL/6J wildtype mice to remove the PhiC31 transgene. (J:146821, J:220523)

Activity:	Tissue activity of this recombinase allele
Driver:	Snap25 (mouse) Summary of all recombinase alleles driven by Snap25. Alliance mouse Snap25 gene page
User Notes:	Additional information submitted by the research community Additional information submitted by the research community Data for AIBS characterization of recombinase activity: \link(Snap25-IRES2-Cre images)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Snap25 Mutation:	26 strains or lines available

Original:	J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76
All:	12 reference(s)