Summary | Transgene origin | Transgene description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Tg(CAG-XBP1*/cre/ERT2)#Grtn
Name:	transgene insertion, Florian R Greten
MGI ID:	MGI:5507828
Synonyms:	Xbp1s-creERT2
Transgene:	Tg(CAG-XBP1*/cre/ERT2)#Grtn  Location: unknown
Alliance:	Tg(CAG-XBP1*/cre/ERT2)#Grtn page

Strain of Origin:

Not Specified

Transgene Type:    Transgenic (Inducible, Recombinase) Inducer:    cellular stress and tamoxifen Mutation:    Insertion   Tg(CAG-XBP1*/cre/ERT2)#Grtn expression driven by 1 gene Transgene expression driven by: Organism Driver Gene Note chicken CAG   Mutation details: The pCAX-F-XBP1/deltaDBD-venus plasmid construct was modified by replacing the venus cDNA with cDNA encoding a cre/ERT2 fusion protein. The final construct consists of nucleotides 410-633 of a human XBP1 cDNA including a Kozak sequence and FLAG-tag coding sequence fused to cre/ERT2 cDNA under control of a CAG promoter construct. The XBP1 fragment lacks the DNA binding domain but includes a 26-nt endoplasmic reticulum stress-specific intron. Under normal conditions, the mRNA of the fusion gene is not spliced and translation terminates at a stop codon near the junction of XBP1 and cre/ERT2. During ER stress, the 26 nt intron is spliced out and the XBP1/cre/ERT2 mRNA is transcribed. Founder and line number information is not available. (J:197481)

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available

Original:	J:197481 Schwitalla S, et al., Intestinal tumorigenesis initiated by dedifferentiation and acquisition of stem-cell-like properties. Cell. 2013 Jan 17;152(1-2):25-38
All:	2 reference(s)