Probe:	Fgf14 cDNA3
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS17: embryo	Absent

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	2A/B	Expression was detected in the supraoptic area, septal area, the ventral lining of the Aqueduct of Sylvius and the fourth ventricle.
TS20: spinal cord	Present		2A/B
TS20: thalamus	Present		2A/B
TS20: thymus primordium	Present		2A/B

Structure	Level	Pattern	Image	Note
TS22: brain	Present	Regionally restricted	2C/D	At E14.5 expression in the brain persisted at higher levels in every domain found at E12.5. Expression was broader and more intense in the thickened regions of the subventricular zone adjacent to the neuroepithelium.
TS22: hypothalamus	Present		2C/D
TS22: medulla oblongata	Present		2C/D
TS22: pons	Present		2C/D

Structure	Level	Pattern	Image	Note
TS22: arch of aorta	Strong		2E/F
TS22: ascending aorta	Strong		2E/F
TS22: dorsal aorta	Strong		2E/F
TS22: thymus primordium	Strong		2E/F

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present		2G/H , 3A/B
TS22: medulla oblongata	Present		3C/D , 2G/H
TS22: pons	Present		3C/D , 2G/H

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Graded	2I/J	Expression was detected in a ventral to dorsal gradient, with no expression in the dorsal-most regions.
TS22: spinal cord ventricular layer	Absent		2I/J

Structure	Level	Pattern	Image	Note
TS22: arch of aorta	Present		2K/L
TS22: ascending aorta	Present		2K/L
TS22: dorsal aorta	Present		2K/L

Structure	Level	Pattern	Image	Note
TS22: dorsal aorta	Strong		2M/N
TS22: pulmonary artery	Strong		2M/N

Structure	Level	Pattern	Image	Note
TS22: common carotid artery	Strong		2O/P
TS22: thymus primordium	Strong		2O/P

Structure	Level	Pattern	Image	Note
TS24: thymus	Present			Expression was below detectable levels in all tissues with the exception of the thymus.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	4A Fgf14 E12.5	Expression was detected in the subventricular zone around the fourth ventricle, but not in the dorsal edges.

Structure	Level	Pattern	Image	Note
TS24: cerebellum	Absent		4A Fgf14 E16.5	No expression was detected in the external germinal layer.

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Weak	Regionally restricted	4A Fgf14 p1	Expression was detected in the innermost layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present	Regionally restricted	4A Fgf14 p4 , 4B Fgf14	Expression was detected in the deep regions.

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Strong		4A Fgf14 p16

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Strong		4A Fgf14 p30