Antibody:	Anti-AQP9
Detection system:	Secondary antibody coupled to rhodamine

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Weak		3a	Low level membrane-associated immunofluorescence was detected.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Ambiguous		3b	No staining was observed in membrane regions.

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present		3c	Membrane-associated immunofluorescence was observed at cell-cell contacts.

Structure	Level	Pattern	Image	Note
TS4: compacted morula	Present		3d	Expression was detected in the outer blastomeres of the compacted morula. There was some evidence of perinuclear staining within the cytoplasm of individual blastomeres.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		3e	Expression was detected within the cytoplasm of inner cell mass cells.
TS4: mural trophectoderm	Present		3e	Expression in the apical membrane domains of mural and polar trophectoderm.
TS4: polar trophectoderm	Present		3e	Expression in the apical membrane domains of mural and polar trophectoderm.