Gene Expression Data
Assay Details
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Assay
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Reference:
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J:82847
Barcroft LC, et al., Aquaporin proteins in murine trophectoderm mediate transepithelial water movements during cavitation. Dev Biol. 2003 Apr 15;256(2):342-54
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5505886
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Gene symbol:
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Aqp3
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Gene name:
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aquaporin 3
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Antibody:
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Anti-AQP3
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Detection system:
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Secondary antibody coupled to rhodamine
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Results
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Specimen
1a:
embryonic day 0.5
(more  )
Note: One-cell embryo.
Specimen
1a:
(close  )
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Genetic Background:
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involves: C57BL/6 * CD-1
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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One-cell embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Present |
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1a
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Expression was present within the cytoplasm. No staining was observed in membrane regions.
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Specimen
1b:
embryonic day 1.0
(more )
Note: Two-cell embryo.
Specimen
1b:
(close )
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Genetic Background:
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involves: C57BL/6 * CD-1
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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Two-cell embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
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1b
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Expression was present within the cytoplasm. No staining was observed in membrane regions.
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Specimen
1c:
embryonic day 2.5
(more )
Note: Eight-cell embryo.
Specimen
1c:
(close )
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Genetic Background:
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involves: C57BL/6 * CD-1
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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Eight-cell embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 8-cell stage embryo |
Present |
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1c
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Expression was evident in the blastomeres, where distinct foci of fluorescence were seen throughout the cytoplasm of all embryonic cells. No staining was observed in membrane regions.
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Specimen
1d:
embryonic day 3.0
(more )
Note: Morula.
Specimen
1d:
(close )
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Genetic Background:
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involves: C57BL/6 * CD-1
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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Morula.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Present |
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1d
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Expression was detected in an apolar fashion within the membrane domains of all blastomeres of the compacted morula.
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Specimen
1e:
embryonic day 3.5
(more )
Note: Blastocyst.
Specimen
1e:
(close )
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Genetic Background:
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involves: C57BL/6 * CD-1
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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Blastocyst.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: inner cell mass |
Present |
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1e
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An apolar distribution of membrane-associated expression was maintained within the inner cell mass.
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| TS4: trophectoderm |
Present |
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1e
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Expression in the trophectoderm was restricted to the basolateral cell margins.
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Analysis Tools