Pbx1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:82126 Schnabel CA, et al., Pbx1 regulates nephrogenesis and ureteric branching in the developing kidney. Dev Biol. 2003 Feb 15;254(2):262-76
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5505254
Gene symbol:	Pbx1
Gene name:	pre B cell leukemia homeobox 1

Antibody:	anti-Pbx1b
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Results

2A ©

Specimen 2A: embryonic day 11.0 (more

)

Structure	Level	Pattern	Image	Note
TS18: metanephric mesenchyme	Present		2A	Expression is in the metanephric blastema and surrounding mesenchyme.
TS18: intraembryonic coelom	Present	Regionally restricted	2A	Expression is in coelomic epithelium.
TS18: ureteric bud	Absent		2A

2B ©

Specimen 2B: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: peripheral blastema	Strong		2B	Expression is in the metanephric blastema and surrounding mesenchyme.
TS19: mesenchyme of metanephros	Strong		2B	Expression is in the metanephric blastema and surrounding mesenchyme.

2C ©

Specimen 2C: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: mesenchyme of metanephros	Present	Regionally restricted	2C	Expression is thoughout the mesenchymal compartment in both the cortex and medulla.

2D ©

Specimen 2D: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: mesenchyme of metanephros	Present	Regionally restricted	2D	Expression is thoughout the mesenchymal compartment in both the cortex and medulla

2E ©

Specimen 2E: embryonic day 14.0 (more

)

Structure	Level	Pattern	Image	Note
TS22: nephrogenic zone	Weak		2E	Expression is in pretubular aggregates and cells undergoing epithelial conversion.
TS22: maturing glomerular tuft	Present		2E

2F ©

Specimen 2F: embryonic day 14.0 (more

)

Structure	Level	Pattern	Image	Note
TS22: mesenchyme of metanephros	Present		2F	Higher levels are seen in stromal cells and lower levels are in the induced mesenchyme.

2G ©

Specimen 2G: embryonic day 13.5; Pbx1^tm1Mlc/Pbx1^tm1Mlc (more

)

Structure	Level	Pattern	Image	Note
TS21: metanephros	Absent		2G

2J ©

Specimen 2J: embryonic day 11.0 (more

)

Structure	Level	Pattern	Image	Note
TS18: metanephric mesenchyme	Present		2J

5G ©

Specimen 5G: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: mesenchyme of metanephros	Present		5G

5H ©

Specimen 5H: embryonic day 13.5; Bmp7^tm1Rob/Bmp7^tm1Rob (more

)

Structure	Level	Pattern	Image	Note
TS21: mesenchyme of metanephros	Present		5H	Expression in this mutant is the same as in wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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