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Gene Expression Data
Assay Details
Assay
Reference: J:86839 Liguori GL, et al., Anterior neural plate regionalization in cripto null mutant mouse embryos in the absence of node and primitive streak. Dev Biol. 2003 Dec 15;264(2):537-49
Assay type: RNA in situ
MGI Accession ID: MGI:5504410
Gene symbol: Irx3
Gene name: Iroquois related homeobox 3
Probe: Irx3 cDNA2
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 5A: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS13: future prosencephalon Present Regionally restricted 5A Expression was detected in caudal diencephalon.
TS13: future hindbrain Present 5A
TS13: future midbrain Present 5A
TS13: future spinal cord Present 5A
Specimen 5A': embryonic day 8.5; Criptotm1Eda/Criptotm1Eda (more )
Structure Level Pattern Image Note
TS13: embryo Present Regionally restricted 5A' Expression was detected in the intermediate region of the mutant embryo.
Specimen Table 1: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS13: future prosencephalon Present Regionally restricted Expression was detected in the diencephalon.
TS13: future midbrain Present
TS13: midbrain-hindbrain junction Present
TS13: future hindbrain Present Regionally restricted Expression was detected in anterior rhombencephalon.
Specimen Table 1: embryonic day 8.5; Criptotm1Eda/Criptotm1Eda (more )
Structure Level Pattern Image Note
TS13: future prosencephalon Present Regionally restricted Expression was detected in the diencephalon.
TS13: future midbrain Present
TS13: midbrain-hindbrain junction Present
TS13: future hindbrain Present Regionally restricted Expression was detected in anterior rhombencephalon.
Specimen 7C: embryonic day 8.5 (more )
Note: Double labeled: blue - Irx3; red - Six3 (assay MGI:5504436)
Structure Level Pattern Image Note
TS13: future prosencephalon Present Regionally restricted 7C Expression was detected in caudal diencephalon.
TS13: future midbrain Present 7C
Specimen 7C': embryonic day 8.5; Criptotm1Eda/Criptotm1Eda (more )
Note: Double labeled: blue - Irx3; red - Six3 (assay MGI:5504436)
Structure Level Pattern Image Note
TS13: future midbrain Present Regionally restricted 7C' Staining was maintained in a nested pattern with Six3 in the mutant embryo.
TS13: future prosencephalon Present Regionally restricted 7C' Staining was maintained in a nested pattern with Six3 in the mutant embryo.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory