Antibody:	p57kip2 antibody (M-20; sc-1039)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Authors report similar staining patterns using the E-17 antibody (MGI:5503027).

Structure	Level	Pattern	Image	Note
TS21: lung epithelium	Present	Regionally restricted	1A	Expression in bronchiole epithelium cells. Weak expression in lung interstitial cells.
TS21: rib cartilage condensation	Present	Regionally restricted	1A	Expression in rib cartilage chondrocytes.

Structure	Level	Pattern	Image	Note
TS17: heart	Present		2A

Structure	Level	Pattern	Image	Note
TS19: heart	Present		2B

Structure	Level	Pattern	Image	Note
TS21: endocardial tissue	Present	Regionally restricted	2C	Expression restricted to endocardium of atria and ventricles.

Structure	Level	Pattern	Image	Note
TS21: atrium endocardial lining	Present		2D

Structure	Level	Pattern	Image	Note
TS21: rib cartilage condensation	Strong	Widespread	2E	Strong expression in chondrocytes throughout length of rib cartilage including the hypertrophic zone.
TS21: trunk mesenchyme	Present	Regionally restricted	2E	Expression in perichondrium.

Structure	Level	Pattern	Image	Note
TS25: rib	Present	Regionally restricted	2F	Expresssion in cartilage restricted to hypertrophic zone.
TS25: intercostal muscle	Present	Widespread	2F

Structure	Level	Pattern	Image	Note
TS21: rib cartilage condensation	Strong	Regionally restricted	2G	Strong expression in chondrocytes throughout length of rib cartilage including the hypertrophic zone.
TS21: trunk mesenchyme	Present	Regionally restricted	2G	Expression in perichondrium.

Structure	Level	Pattern	Image	Note
TS23: rib	Present	Regionally restricted	2H	Expression restricted to cartilage. No expression in perichondrium

Structure	Level	Pattern	Image	Note
TS23: anterior abdominal wall muscle	Present	Patchy	2I	Patchy expression in skeletal muscle, especially abdominal wall muscles.

Structure	Level	Pattern	Image	Note
TS25: body wall	Present	Regionally restricted	2J	Expression in nuclei and cytoplasm of some cells in body wall muscles, but not in others.

Structure	Level	Pattern	Image	Note
TS25: body wall	Present	Regionally restricted	2K	Expression in nuclei and cytoplasm of some cells in body wall muscles, but not in others.

Structure	Level	Pattern	Image	Note
TS19: infundibular recess of 3rd ventricle	Strong		3A
TS19: Rathke's pouch	Present		3A
TS19: central nervous system	Weak	Regionally restricted	3A	Lower expression in neural fold.

Structure	Level	Pattern	Image	Note
TS19: infundibular recess of 3rd ventricle	Strong		3B	Strongest expression in anterior infundibulum.
TS19: Rathke's pouch	Present		3B

Structure	Level	Pattern	Image	Note
TS21: pituitary gland	Present		3C

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Present	Regionally restricted	3D	Expression in neural fold, most evident in peripheral cells.

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Present	Regionally restricted	3E	Expression in neural fold, most evident in peripheral cells.

Structure	Level	Pattern	Image	Note
TS21: choroid plexus	Present		3F

Structure	Level	Pattern	Image	Note
TS21: meninges	Present		3G	Expression in leptomeninges.

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Absent		3H

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present
TS21: eye	Present	Regionally restricted
TS21: retina	Present
TS21: thyroid gland	Present	Regionally restricted		Expression in mesenchyme and proximal duct region.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: Sertoli cell	Sertoli cell	Present	Regionally restricted	4A	Expression in presumptive sertoli cell precursors in the testes.
TS21: pancreas parenchyma		Present		4A	Expression in interstitial mesenchymal cells in the pancreas.

Structure	Level	Pattern	Image	Note
TS22: testis	Present	Single cells		Expression in testes restricted a a few cells dividing the gonad from the developing kidney.

Structure	Level	Pattern	Image	Note
TS21: adrenal gland	Present	Regionally restricted	4B	Expression in adrenal in some but not all cells of both epithelial (duct) and interstitial layers.

Structure	Level	Pattern	Image	Note
TS21: adrenal gland	Present	Single cells	4C	Expression in a mitotic cell in the developing adrenal gland.

Structure	Level	Pattern	Image	Note
TS21: semicircular canal epithelium	Strong	Regionally restricted	4D	Strong expression in epithelial cells of semicircular canals.
TS21: posterior semicircular canal	Very strong	Regionally restricted	4D	Particularly strong at inside corners of posterior and anterior semicircular canals.
TS21: superior semicircular canal	Very strong	Regionally restricted	4D	Particularly strong at inside corners of posterior and anterior semicircular canals.

Structure	Level	Pattern	Image	Note
TS25: inner ear	Absent		4E
TS25: semicircular canal	Absent		4E

Structure	Level	Pattern	Image	Note
TS21: nasal cavity	Present	Regionally restricted	4F
TS21: olfactory epithelium	Strong		4F
TS21: nose	Strong	Regionally restricted	4F	Strong expression in cartilage primordia of turbinate bones.

Structure	Level	Pattern	Image	Note
TS21: tooth mesenchyme	Strong	Regionally restricted	4G	Strong expression in ectomesenchyme and majority of mesenchymal cells surrounding the emerging tooth.
TS21: tooth epithelium	Present	Regionally restricted	4G	Expression in epithelium restricted to thickened marginal regions.
TS21: enamel knot	Absent		4G

Structure	Level	Pattern	Image	Note
TS21: tooth mesenchyme	Present	Regionally restricted	4H	Expression in ectomesenchyme. Once the cust is formed, expression in mesenchymal cells diminishes. Weak expression in some cells in the pre-odontoblast layer.
TS21: enamel knot	Absent		4H

Structure	Level	Pattern	Image	Note
TS21: gut epithelium	Strong	Regionally restricted	4I	Strong expression in endodermal layer of gut and stomach. Weak expression in epithelial cells surrounding gut lumen,
TS21: gut mesenchyme	Weak		4I

Structure	Level	Pattern	Image	Note
TS25: colon epithelium	Present	Regionally restricted	4J	Expression in colon restricted to epithelial villi cells.

Structure	Level	Pattern	Image	Note
TS21: metanephros	Present	Graded	5A

Structure	Level	Pattern	Image	Note
TS21: mesenchyme of metanephros	Strong	Regionally restricted	5B	Strong expression in condensed epithelializing mesenchymal cells and in stromal mesenchymal cells surrounding epithelial tubules.
TS21: renal tubule	Present	Regionally restricted	5B	Less intense expression in developing proximal tubules.

Structure	Level	Pattern	Image	Note
TS21: mesenchyme of metanephros	Strong	Regionally restricted	5C	Intense expression in stromal mesenchymal cells adjacent to tubules and condensed epithelial mesenchyme.
TS21: renal tubule	Present	Regionally restricted	5C	Lower level of expression in most proximal portions of S and comma tubules.

Structure	Level	Pattern	Image	Note
TS23: renal medullary interstitium	Present	Regionally restricted	5D	Expression declined; predominantly restricted to interstitial stromal mesenchymal cells in medullary region.
TS23: renal tubule	Weak	Regionally restricted	5D	Very low levels of expression in some tubules.

Structure	Level	Pattern	Image	Note
TS25: renal proximal tubule	Present	Regionally restricted	5E	Weak expression with exception of some proximal tubules.

Structure	Level	Pattern	Image	Note
TS25: renal proximal convoluted tubule	Present	Regionally restricted	5F	Expression in some rounded cells in proximal convoluted tubules.

Structure	Level	Pattern	Image	Note
TS19: main bronchus epithelium	Present	Regionally restricted	6A	Expression in columnar cells of major bronchi.

Structure	Level	Pattern	Image	Note
TS21: lobar bronchus epithelium	Present	Regionally restricted	6B	Expression in columnar epithelial cells in major and segmental bronchi.
TS21: main bronchus epithelium	Present	Regionally restricted	6B	Expression in columnar epithelial cells in major and segmental bronchi.

Structure	Level	Pattern	Image	Note
TS21: lung mesenchyme	Present	Regionally restricted	6C	Expression in mesenchymal stromal overlying cuboidal epithelium.
TS21: lung epithelium	Present	Regionally restricted	6C	No expression in differentiated cuboidal epithelial cells at corners of extending bronchioles.

Structure	Level	Pattern	Image	Note
TS23: lung mesenchyme	Present	Regionally restricted	6D	Expression in mesenchymal stromal overlying cuboidal epithelium.
TS23: lung epithelium	Present	Regionally restricted	6D	No expression in differentiated cuboidal epithelial cells at corners of extending bronchioles.

Structure	Level	Pattern	Image	Note
TS23: lobar bronchus epithelium	Weak	Regionally restricted	6E	Very reduced level of expression, restricted to columnar cells of segmental and terminal bronchi.
TS23: lung	Present	Regionally restricted	6E	No expression in alveoli.

Structure	Level	Pattern	Image	Note
TS25: liver	Absent		6F
TS25: lung	Absent		6F
TS25: diaphragm	Strong		6F

Structure	Level	Pattern	Image	Note
TS19: apical ectodermal ridge	Present		7A
TS19: limb mesenchyme	Present	Regionally restricted	7A	Expression in majority of mesenchymal cells in proximal region of limb bud. No expression in broad band of cells at the distal tip of the bud, encompassing some but not all dividing cells in the progress zone.

Structure	Level	Pattern	Image	Note
TS21: limb cartilage condensation	Present	Regionally restricted	7B	Expression in cartilage and pre-muscle mesenchymal cells.
TS21: limb mesenchyme	Present	Regionally restricted	7B	Expression in cartilage and pre-muscle mesenchymal cells.
TS21: limb digit	Present	Regionally restricted	7B	No expression in a broad region of cells at the tip of each digit.

Structure	Level	Pattern	Image	Note
TS23: limb mesenchyme	Present	Regionally restricted	7C	Very restricted expression in cartilage and muscle.
TS23: limb musculature	Present	Regionally restricted	7C	Very restricted expression in cartilage and muscle.
TS23: limb digit	Present		7C

Structure	Level	Pattern	Image	Note
TS23: limb mesenchyme	Present	Regionally restricted	7D	Very restricted expression in cartilage and muscle.
TS23: limb musculature	Present		7D	Very restricted expression in cartilage and muscle.

Structure	Level	Pattern	Image	Note
TS23: limb digit	Present	Regionally restricted	7E	Expression in apoptosing cells.

Structure	Level	Pattern	Image	Note
TS23: limb digit	Present	Regionally restricted	7F	No expression in ball of cells at each digit tip, separated from band of dividing non-expressing cells by a band of expressing cells.

Structure	Level	Pattern	Image	Note
TS13: extraembryonic component	Present	Regionally restricted	8A	Expression in embryonic contribution to placenta.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Single cells	8A	Expression in a few cells in the maternal decidua.

Structure	Level	Pattern	Image	Note
TS21: extraembryonic component	Present	Regionally restricted	8B	Expression in embryo-derived placenta.

Structure	Level	Pattern	Image	Note
TS25: extraembryonic component	Present	Regionally restricted	8C	Expression in all major layers of embryo-derived placenta, particularly the labyrinth and yolk sac.
TS25: labyrinthine zone	Present		8C
TS25: yolk sac	Present		8C

Structure	Level	Pattern	Image	Note
TS21: trophoblast giant cell	Strong		8D	Strong expression in glycogen cells and trophoblast giant cells.

Structure	Level	Pattern	Image	Note
TS21: yolk sac endoderm	Strong		8E	Strong expression in approximately 50% of yolk sac epithelial cells.

Structure	Level	Pattern	Image	Note
TS21: amnion	Strong		8F	Strong expression in approximately 50% of amnion cells.