Trib2tm1.1(cre/ERT2)Hze
Targeted Allele Detail
|
Symbol: |
Trib2tm1.1(cre/ERT2)Hze |
Name: |
tribbles pseudokinase 2; targeted mutation 1.1, Hongkui Zeng |
MGI ID: |
MGI:5501236 |
Synonyms: |
Trib2-2A-CreERT2, Trib2-2A-CreERT2-DeltaNeo/Hygro |
Gene: |
Trib2 Location: Chr12:15841728-15866923 bp, - strand Genetic Position: Chr12, 7.25 cM
|
Alliance: |
Trib2tm1.1(cre/ERT2)Hze page
|
|
|
Allele Type: |
|
Targeted (Inducible, Recombinase) |
Inducer: |
|
tamoxifen |
Mutations: |
|
Insertion, Intragenic deletion
|
|
|
Trib2tm1.1(cre/ERT2)Hze expression driven by
1 gene
Knock-in expression driven by:
|
|
|
Mutation details: ES cells were re-targeted with an "F2A-CreERT2" vector and an FLP-expressing plasmid to facilitate recombination. Correctly re-targeted ES cells had (from 5'to3') an frt3 site within Trib 2 intron 1, a self-cleaving 2A peptide sequence designed to splice a cre/ERT2 fusion gene into a frame with the Trib 2 exon 1 sequence, a bovine growth hormone polyA signal, an AttB site, a PGK-hygromycin-SV40polyA cassette (with a splice donor-frt5 site-RNA splice acceptor in the hygromycin gene), an AttP site, and Trib 2 exon 2 deleted. The resulting chimeric animals were bred to PhiC31-expressing mice to delete the AttB/AttP-flanked sequences and replace them with the recombined AttB/AttP site (AttL).
(J:199218, J:220523)
|
|
|
Activity: |
Tissue activity of this recombinase allele
|
Driver:
|
Trib2
(mouse)
|
|
|
|
|
Original: |
J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76 |
All: |
11 reference(s) |
|