Bcl2l1 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:86668 Kasai S, et al., Haploinsufficiency of Bcl-x leads to male-specific defects in fetal germ cells: differential regulation of germ cell apoptosis between the sexes. Dev Biol. 2003 Dec 1;264(1):202-16
Assay type:	Northern blot
MGI Accession ID:	MGI:5500013
Gene symbol:	Bcl2l1
Gene name:	BCL2-like 1

Probe:	Bcl-xL cDNA2
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Phosphorimaging

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
Brain	P adult	TS28: brain	Present (a)	20 µg; total RNA	Not Specified		Not Specified
Thymus	P adult	TS28: thymus	Present (a)	20 µg; total RNA	Not Specified		Not Specified
Liver	P adult	TS28: liver	Absent (b)	20 µg; total RNA	Not Specified		Not Specified
Spleen	P adult	TS28: spleen	Absent (b)	20 µg; total RNA	Not Specified		Not Specified
Kidney	P adult	TS28: metanephros	Present (a)	20 µg; total RNA	Not Specified		Not Specified
Testis	P adult	TS28: testis	Present (c)	20 µg; total RNA	Not Specified		Male
Ovary	P adult	TS28: ovary	Present (a)	20 µg; total RNA	Not Specified		Female
Brain	P adult	TS28: brain	Present (a)	20 µg; total RNA	involves: C57BL/6	Bcl2l1^tm1Dlo/Bcl2l1⁺	Not Specified
Thymus	P adult	TS28: thymus	Present (a)	20 µg; total RNA	involves: C57BL/6	Bcl2l1^tm1Dlo/Bcl2l1⁺	Not Specified
Liver	P adult	TS28: liver	Absent (b)	20 µg; total RNA	involves: C57BL/6	Bcl2l1^tm1Dlo/Bcl2l1⁺	Not Specified
Spleen	P adult	TS28: spleen	Absent (b)	20 µg; total RNA	involves: C57BL/6	Bcl2l1^tm1Dlo/Bcl2l1⁺	Not Specified
Kidney	P adult	TS28: metanephros	Present (a)	20 µg; total RNA	involves: C57BL/6	Bcl2l1^tm1Dlo/Bcl2l1⁺	Not Specified
Testis	P adult	TS28: testis	Present (d)	20 µg; total RNA	involves: C57BL/6	Bcl2l1^tm1Dlo/Bcl2l1⁺	Male
Ovary	P adult	TS28: ovary	Present (a)	20 µg; total RNA	involves: C57BL/6	Bcl2l1^tm1Dlo/Bcl2l1⁺	Female

Notes:
(a) Present assigned by curator.
(b) Absent assigned by curator.
(c) The authors stated that the transcript was longer in testis compared to other tissues but did not detail which tissues nor size.
(d) The authors stated that the transcript was longer in testis compared to other tissues but did not detail which tissues nor size. Amounts were approximately half compared to wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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