Gene Expression Data
Assay Details
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Assay
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Reference:
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J:86667
Mic FA, et al., Patterning of forelimb bud myogenic precursor cells requires retinoic acid signaling initiated by Raldh2. Dev Biol. 2003 Dec 1;264(1):191-201
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5499875
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Gene symbol:
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Met
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Gene name:
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met proto-oncogene
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Results
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Specimen
1C:
embryonic day 10.5
(more  )
Specimen
1C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
1C
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Distinct dorsal and ventral domains of expression, with no expression in the central region.
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| TS17: dermomyotome |
Present |
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1C
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Specimen
1D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Aldh1a2tm1Gdu/Aldh1a2tm1Gdu
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
1D
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Single expression domain across the dorsoventral axis, shifted to the anterior margin.
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| TS17: dermomyotome |
Present |
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1D
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Expression in dermomyotome not affected in mutant compared with wild-type embryo.
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Specimen
2G:
embryonic day 10.5
(more )
Specimen
2G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Embedding:
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Agarose
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Specimen
2H:
embryonic day 10.5
(more )
Specimen
2H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Strong |
Regionally restricted |
2H
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Highest levels in the center of the anteroposterior axis, with separate dorsal and ventral domains.
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Specimen
2I:
embryonic day 10.5
(more )
Specimen
2I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Embedding:
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Agarose
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Specimen
2J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Aldh1a2tm1Gdu/Aldh1a2tm1Gdu
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
2J
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Authors state that expression is skewed to anterior side compared with wild-type embryos.
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Specimen
2K:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Aldh1a2tm1Gdu/Aldh1a2tm1Gdu
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Weak |
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2K
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No distinct dorsal or ventral domains.
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Specimen
2L:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Aldh1a2tm1Gdu/Aldh1a2tm1Gdu
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Embedding:
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Agarose
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Specimen
3A:
embryonic day 11.5
(more )
Specimen
3A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
3A
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Expression spread out across anteroposterior and proximodistal axes. Highest levels in center of anteroposterior axis, as far distally as the autopod.
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| TS19: hindlimb bud |
Present |
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3A
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Aldh1a2tm1Gdu/Aldh1a2tm1Gdu
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
3B
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Authors report expression limited to an anterior-proximal domain, at an abnormal site. Very little expression in the center, and no expression distally where the autopod would normally be developing.
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| TS19: hindlimb bud |
Present |
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3B
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Authors report expression similar to that seen in wild-type limb buds.
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Specimen
5G:
embryonic day 10.25
(more )
Specimen
5G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.25
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
5G
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Expression is internal, excluded from the outer margin.
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Specimen
5H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Aldh1a2tm1Gdu/Aldh1a2tm1Gdu
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Age:
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embryonic day 10.25
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
5H
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Expression is shifted anteriorly and proximally.
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