Gene Expression Data
Assay Details
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Assay
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Reference:
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J:59068
Pispa J, et al., Cusp patterning defect in Tabby mouse teeth and its partial rescue by FGF. Dev Biol. 1999 Dec 15;216(2):521-34
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5499545
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Gene symbol:
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Fgf4
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Gene name:
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fibroblast growth factor 4
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Probe:
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Fgf-4 cDNA1
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Probe preparation:
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labelled with S35
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Visualized with:
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Autoradiography
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Results
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Specimen
4a:
embryonic day 14.0
(more  )
Specimen
4a:
(close  )
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Genetic Background:
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involves: CBA-T6T6 * NMRI
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lower jaw molar |
Strong |
Regionally restricted |
4a
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Strong expression in the primary enamel knot, extending over 200 micrometers mesiodistally.
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| TS22: primary enamel knot |
Strong |
Regionally restricted |
4a
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Strong expression in the primary enamel knot, extending over 200 micrometers mesiodistally.
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Specimen
4b:
embryonic day 14.0; EdaTa/?
(more )
Note: the mutation is X-linked; the embryo could have been homozygous or hemizygous
Specimen
4b:
(close )
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Genetic Background:
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involves: B6CBACa Aw-J/A-EdaTa/O
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Mutant Allele(s):
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EdaTa/?
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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the mutation is X-linked; the embryo could have been homozygous or hemizygous
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lower jaw molar |
Weak |
Regionally restricted |
4b
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Weaker expression than in wild type, with smaller expression domain, extending only 100 micrometers.
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| TS22: primary enamel knot |
Weak |
Regionally restricted |
4b
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Weaker expression than in wild type, with smaller expression domain, extending only 100 micrometers.
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Specimen
4c:
embryonic day 16.0
(more )
Specimen
4c:
(close )
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Genetic Background:
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involves: CBA-T6T6 * NMRI
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: lower jaw molar |
Present |
Regionally restricted |
4c
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Secondary enamel knots corresponding to future cusp tips are present as small areas of expression.
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| TS24: secondary enamel knot |
Present |
Regionally restricted |
4c
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Secondary enamel knots corresponding to future cusp tips are present as small areas of expression.
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Specimen
4d:
embryonic day 16.0; EdaTa/?
(more )
Note: the mutation is X-linked; the embryo could have been homozygous or hemizygous
Specimen
4d:
(close )
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Genetic Background:
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involves: B6CBACa Aw-J/A-EdaTa/O
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Mutant Allele(s):
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EdaTa/?
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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the mutation is X-linked; the embryo could have been homozygous or hemizygous
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: lower jaw molar |
Present |
Regionally restricted |
4d
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Large mesiodistal expression domain, corresponding to first buccal and lingual secondary knots.
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| TS24: secondary enamel knot |
Present |
Regionally restricted |
4d
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Large mesiodistal expression domain, corresponding to first buccal and lingual secondary knots.
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Analysis Tools