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Gene Expression Data
Assay Details
Assay
Reference: J:54064 Kablar B, et al., Development in the absence of skeletal muscle results in the sequential ablation of motor neurons from the spinal cord to the brain. Dev Biol. 1999 Apr 1;208(1):93-109
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5496161
Gene symbol: Nefm
Gene name: neurofilament, medium polypeptide
Antibody: Anti-NF160
Results
Specimen 6A,C,E: embryonic day 12.5 (more )
Note: Brachial level section.
Structure Level Pattern Image Note
TS20: spinal nerve Present Regionally restricted 6C , 6E , 6A Expression was detected in the spinal nerve.
Specimen 6B,D,F: embryonic day 12.5; Myf5tm1Jae/Myf5tm1Jae, Myod1tm1Jae/Myod1tm1Jae (more )
Note: Brachial level section.
Structure Level Pattern Image Note
TS20: spinal nerve Present Regionally restricted 6B , 6F , 6D Expression was detected in the spinal nerve, but there were phenotypic abnormalities in nerve branches.
Specimen 6G: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: brachial plexus Present 6G Expression was detected in the ventral and dorsal characters of the main forelimb nerve branches.
Specimen 6H: embryonic day 12.5; Myf5tm1Jae/Myf5tm1Jae, Myod1tm1Jae/Myod1tm1Jae (more )
Structure Level Pattern Image Note
TS20: brachial plexus Present 6H Expression was detected as in wild type in the ventral and dorsal characters of the main forelimb nerve branches.
Specimen 6I: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: brachial plexus Present 6I Expression was detected in finer branches of the forelimb nerve.
Specimen 6J: embryonic day 12.5; Myf5tm1Jae/Myf5tm1Jae, Myod1tm1Jae/Myod1tm1Jae (more )
Structure Level Pattern Image Note
TS20: brachial plexus Present 6J Expression was detected as in wild type in the main forelimb nerve, but finer branches to the musculature were absent.
Specimen 6K: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: brachial plexus Present 6K Expression was detected in the cutaneous branches of the forelimb nerve.
Specimen 6L: embryonic day 12.5; Myf5tm1Jae/Myf5tm1Jae, Myod1tm1Jae/Myod1tm1Jae (more )
Structure Level Pattern Image Note
TS20: brachial plexus Present 6L Expression was detected as in wild type in the cutaneous branches of the forelimb nerve.
Specimen not shown E15.5 WT: embryonic day 15.5 (more )
Note: Transverse section of the upper thoracic spinal cord.
Structure Level Pattern Image Note
TS23: dorsal root ganglion Present Expression was detected in neurons in the dorsal root ganglion.
Specimen not shown E15.5 mutant: embryonic day 15.5; Myf5tm1Jae/Myf5tm1Jae, Myod1tm1Jae/Myod1tm1Jae (more )
Note: Transverse section of the upper thoracic spinal cord.
Structure Level Pattern Image Note
TS23: dorsal root ganglion Present The number of labeled neurons was somewhat decreased in the dorsal root ganglion of the mutant.
Specimen not shown E16.5 WT: embryonic day 16.5 (more )
Note: Transverse section of the upper thoracic spinal cord.
Structure Level Pattern Image Note
TS24: dorsal root ganglion Present Expression was detected in neurons in the dorsal root ganglion. There were somewhat fewer neurons labeled than at E15.5.
Specimen not shown E16.5 mutant: embryonic day 16.5; Myf5tm1Jae/Myf5tm1Jae, Myod1tm1Jae/Myod1tm1Jae (more )
Note: Transverse section of the upper thoracic spinal cord.
Structure Level Pattern Image Note
TS24: dorsal root ganglion Present The number of labeled neurons was somewhat decreased in the dorsal root ganglion of the mutant.
Specimen 7A: embryonic day 17.5 (more )
Note: Transverse section of the upper thoracic spinal cord.
Structure Level Pattern Image Note
TS25: dorsal root ganglion Present 7A Expression was detected in large and small neurons in the dorsal root ganglion.
Specimen 7B: embryonic day 17.5; Myf5tm1Jae/Myf5tm1Jae, Myod1tm1Jae/Myod1tm1Jae (more )
Note: Transverse section of the upper thoracic spinal cord.
Structure Level Pattern Image Note
TS25: dorsal root ganglion Present 7B There were markedly decreased numbers of large neurons in the dorsal root ganglion of the mutant, but the number of small neurons was similar to wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory