Sry RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:58863 Nagamine CM, et al., Sex reversal caused by Mus musculus domesticus Y chromosomes linked to variant expression of the testis-determining gene Sry. Dev Biol. 1999 Dec 1;216(1):182-94
Assay type:	RT-PCR
MGI Accession ID:	MGI:5495124
Gene symbol:	Sry
Gene name:	sex determining region of Chr Y

Probe:	Sry-pC, Sry-pD
Probe preparation:	labelled with P33
Visualized with:	Autoradiography
Assay notes:	Data was shown in a ratio plot figure. Lim1 (Lhx1, MGI:99783) was used as an internal control.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	612 bp	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
FVB 8	E10.5 (a)	TS17: gonad primordium TS17: mesonephros	Absent	Not Specified; total RNA	C57BL/6-Chr Y^FVB		Male	8 tail somites embryos.
FVB 9-10	E11.0 (a)	TS18: gonad primordium TS18: mesonephros	Present	Not Specified; total RNA	C57BL/6-Chr Y^FVB		Male	9-10 tail somites embryos.
FVB 11-12	E11.5 (a)	TS19: gonad TS19: mesonephros	Present	Not Specified; total RNA	C57BL/6-Chr Y^FVB		Male	11-12 tail somites embryos.
FVB 13-14	E11.5 (a)	TS19: gonad TS19: mesonephros	Present	Not Specified; total RNA	C57BL/6-Chr Y^FVB		Male	13-14 tail somites embryos.
FVB 15-16	E11.5 (a)	TS19: gonad TS19: mesonephros	Present	Not Specified; total RNA	C57BL/6-Chr Y^FVB		Male	15-16 tail somites embryos.
FVB 17-18	E11.5 (a)	TS19: gonad TS19: mesonephros	Present (b)	Not Specified; total RNA	C57BL/6-Chr Y^FVB		Male	17-18 tail somites embryos.
FVB 19-20	E11.5 (a)	TS19: gonad TS19: mesonephros	Present	Not Specified; total RNA	C57BL/6-Chr Y^FVB		Male	19-20 tail somites embryos.
FVB 21-22	E12.5 (a)	TS20: mesonephros TS20: testis	Present	Not Specified; total RNA	C57BL/6-Chr Y^FVB		Male	21-22 tail somites embryos.
FVB 23-24	E12.5 (a)	TS20: mesonephros TS20: testis	Present	Not Specified; total RNA	C57BL/6-Chr Y^FVB		Male	23-24 tail somites embryos.
TIR 8	E10.5 (a)	TS17: gonad primordium TS17: mesonephros	Absent	Not Specified; total RNA	C57BL/6-Chr Y^TIR		Not Specified	Severe sex reversal. 8 tail somites embryos.
TIR 9-10	E11.0 (a)	TS18: gonad primordium TS18: mesonephros	Present	Not Specified; total RNA	C57BL/6-Chr Y^TIR		Not Specified	Severe sex reversal. 9-10 tail somites embryos.
TIR 11-12	E11.5 (a)	TS19: gonad TS19: mesonephros	Present (c)	Not Specified; total RNA	C57BL/6-Chr Y^TIR		Not Specified	Severe sex reversal. 11-12 tail somites embryos.
TIR 13-14	E11.5 (a)	TS19: gonad TS19: mesonephros	Present (c)	Not Specified; total RNA	C57BL/6-Chr Y^TIR		Not Specified	Severe sex reversal. 13-14 tail somites embryos.
TIR 15-16	E11.5 (a)	TS19: gonad TS19: mesonephros	Present (c)	Not Specified; total RNA	C57BL/6-Chr Y^TIR		Not Specified	Severe sex reversal. 15-16 tail somites embryos.
TIR 17-18	E11.5 (a)	TS19: gonad TS19: mesonephros	Present (c)	Not Specified; total RNA	C57BL/6-Chr Y^TIR		Not Specified	Severe sex reversal. 17-18 tail somites embryos.
TIR 19-20	E11.5 (a)	TS19: gonad TS19: mesonephros	Present (d)	Not Specified; total RNA	C57BL/6-Chr Y^TIR		Not Specified	Severe sex reversal. 19-20 tail somites embryos.
TIR 21-22	E12.5 (a)	TS20: gonad TS20: mesonephros	Present	Not Specified; total RNA	C57BL/6-Chr Y^TIR		Not Specified	Severe sex reversal. 21-22 tail somites embryos.
TIR 23-24	E12.5 (a)	TS20: gonad TS20: mesonephros	Present	Not Specified; total RNA	C57BL/6-Chr Y^TIR		Not Specified	Severe sex reversal. 23-24 tail somites embryos.
F1 9-10	E11.0 (a)	TS18: gonad primordium TS18: mesonephros	Present	Not Specified; total RNA	(SWR x C57BL/6-Chr Y^TIR)F1		Male	9-10 tail somites embryos.
F1 11-12	E11.5 (a)	TS19: gonad TS19: mesonephros	Present	Not Specified; total RNA	(SWR x C57BL/6-Chr Y^TIR)F1		Male	11-12 tail somites embryos.
F1 13-14	E11.5 (a)	TS19: gonad TS19: mesonephros	Present (e)	Not Specified; total RNA	(SWR x C57BL/6-Chr Y^TIR)F1		Male	13-14 tail somites embryos.
F1 15-16	E11.5 (a)	TS19: gonad TS19: mesonephros	Present (f)	Not Specified; total RNA	(SWR x C57BL/6-Chr Y^TIR)F1		Male	15-16 tail somites embryos.
F1 17-18	E11.5 (a)	TS19: gonad TS19: mesonephros	Present	Not Specified; total RNA	(SWR x C57BL/6-Chr Y^TIR)F1		Male	17-18 tail somites embryos.
F1 19-20	E11.5 (a)	TS19: gonad TS19: mesonephros	Present	Not Specified; total RNA	(SWR x C57BL/6-Chr Y^TIR)F1		Male	19-20 tail somites embryos.

Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors. Age normalized so that noon of plug day = E0.5.
(b) Highest value of all the tested ages for B6-YFVB.
(c) Values were 30-49% lower of that for B6-YFVB.
(d) Highest value of all the tested ages for B6-YTIR, only 51% of that for B6-YFVB.
(e) Values were significantly greater than those of B6-YTIR.
(f) Values were significantly greater than those of B6-YTIR. Highest value of all the tested ages.

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