Gene Expression Data
Assay Details
Assay
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Reference:
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J:56938
Yuan W, et al., The mouse SLIT family: secreted ligands for ROBO expressed in patterns that suggest a role in morphogenesis and axon guidance. Dev Biol. 1999 Aug 15;212(2):290-306
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5493271
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Gene symbol:
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Slit3
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Gene name:
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slit guidance ligand 3
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Probe:
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mSlit3 probe
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Probe preparation:
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labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3I:
embryonic day 8.25
(more )
Specimen
3I:
(close )
Genetic Background:
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Swiss Webster
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Age:
|
embryonic day 8.25
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Sex:
|
Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Structure |
Level |
Pattern |
Image |
Note |
TS12: neural tube |
Present |
Regionally restricted |
3I
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Expression in ventral spinal cord (neural tube).
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Specimen
3J:
embryonic day 10.5
(more )
Specimen
3J:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Specimen
3K:
embryonic day 11.5
(more )
Specimen
3K:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Specimen
3L:
embryonic day 13.5
(more )
Specimen
3L:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
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Specimen
4I:
embryonic day 8.0
(more )
Specimen
4I:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: neural fold |
Present |
|
4I
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Expression in ventral midline of neural groove (floor plate) and neural fold prior to closure of neural tube.
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TS12: neural plate |
Present |
|
4I
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Expression in ventral midline of neural groove (floor plate) and neural fold prior to closure of neural tube.
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Specimen
4J:
embryonic day 8.5
(more )
Specimen
4J:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: neural plate |
Present |
|
4J
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Expression in ventral midline of neural groove (floor plate.
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TS13: future brain |
Present |
Regionally restricted |
4J
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Expression in the commissural plate.
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Specimen
4K:
embryonic day 9.5
(more )
Specimen
4K:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: floor plate |
Present |
|
4K
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Expression in ventral midline of neural groove (floor plate) after closure of neural tube.
|
TS15: future brain |
Present |
Regionally restricted |
4K
|
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Specimen
4L:
embryonic day 9.5
(more )
Specimen
4L:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5I:
embryonic day 10.5
(more )
Specimen
5I:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5J:
embryonic day 11.5
(more )
Specimen
5J:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: ear |
Strong |
Regionally restricted |
5J
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Prominent expression in otic vesicle.
|
TS19: branchial groove |
Weak |
|
5J
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Decreased expression in branchial clefts.
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TS19: limb mesenchyme |
Present |
Regionally restricted |
5J
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Expression in distal limb bud mesenchyme. Expression most intense in distal anterior mesenchyme and in proximal posterior cleft between limb bud and in the lateral ridge.
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TS19: trunk |
Present |
Regionally restricted |
5J
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No expression in inter-limb lateral ridge tissue.
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Specimen
6B:
embryonic day 11.5
(more )
Specimen
6B:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: forelimb bud |
Present |
Regionally restricted |
6B
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Expression in anterior tip of limb bud.
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Specimen
6E:
embryonic day 13.5
(more )
Specimen
6E:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
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Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: forelimb |
Weak |
Regionally restricted |
6E
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Weak expression in wrist.
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TS21: hand |
Weak |
Regionally restricted |
6E
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Weak expression in superficial layers in palm and in proximal part of digits excluding digit tips.
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Specimen
7C:
embryonic day 15.5
(more )
Specimen
7C:
(close )
Genetic Background:
|
Swiss Webster
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Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
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Specimen
7F:
embryonic day 15.5
(more )
Specimen
7F:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 15.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: trigeminal ganglion |
Present |
Regionally restricted |
7F
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Expression in whisker follicle in outer root sheath along the shaft and in the layer surrounding the bulb.
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Specimen
8C:
embryonic day 14.5
(more )
Specimen
8C:
(close )
Genetic Background:
|
Swiss Webster
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Age:
|
embryonic day 14.5
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Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
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Specimen
8G:
embryonic day 17.5
(more )
Specimen
8G:
(close )
Genetic Background:
|
Swiss Webster
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Age:
|
embryonic day 17.5
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
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