Probe:	mSlit3 probe
Probe preparation:	labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS12: neural tube	Present	Regionally restricted	3I	Expression in ventral spinal cord (neural tube).

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		3J
TS17: neural tube lateral wall	Present	Regionally restricted	3J	Expression in motor columns.

Structure	Level	Pattern	Image	Note
TS19: neural tube floor plate	Present		3K
TS19: neural tube lateral wall	Present	Regionally restricted	3K	Expression in motor columns.

Structure	Level	Pattern	Image	Note
TS21: spinal cord floor plate	Present		3L
TS21: basal columns	Present		3L	Expression in motor columns.

Structure	Level	Pattern	Image	Note
TS12: neural fold	Present		4I	Expression in ventral midline of neural groove (floor plate) and neural fold prior to closure of neural tube.
TS12: neural plate	Present		4I	Expression in ventral midline of neural groove (floor plate) and neural fold prior to closure of neural tube.

Structure	Level	Pattern	Image	Note
TS13: neural plate	Present		4J	Expression in ventral midline of neural groove (floor plate.
TS13: future brain	Present	Regionally restricted	4J	Expression in the commissural plate.

Structure	Level	Pattern	Image	Note
TS15: floor plate	Present		4K	Expression in ventral midline of neural groove (floor plate) after closure of neural tube.
TS15: future brain	Present	Regionally restricted	4K

Structure	Level	Pattern	Image	Note
TS15: 1st branchial groove	Present		4L
TS15: 2nd branchial groove	Present		4L
TS15: otocyst	Present		4L

Structure	Level	Pattern	Image	Note
TS17: otocyst	Strong		5I	Prominent expression in otic vesicle.
TS17: branchial groove	Weak		5I	Decreased expression in branchial clefts.
TS17: limb mesenchyme	Present	Regionally restricted	5I	Expression in distal limb bud mesenchyme.
TS17: trunk	Present	Regionally restricted	5I	Expresssion in lateral ridge tissue flanking the limb bud.

Structure	Level	Pattern	Image	Note
TS19: ear	Strong	Regionally restricted	5J	Prominent expression in otic vesicle.
TS19: branchial groove	Weak		5J	Decreased expression in branchial clefts.
TS19: limb mesenchyme	Present	Regionally restricted	5J	Expression in distal limb bud mesenchyme. Expression most intense in distal anterior mesenchyme and in proximal posterior cleft between limb bud and in the lateral ridge.
TS19: trunk	Present	Regionally restricted	5J	No expression in inter-limb lateral ridge tissue.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	6B	Expression in anterior tip of limb bud.

Structure	Level	Pattern	Image	Note
TS21: forelimb	Weak	Regionally restricted	6E	Weak expression in wrist.
TS21: hand	Weak	Regionally restricted	6E	Weak expression in superficial layers in palm and in proximal part of digits excluding digit tips.

Structure	Level	Pattern	Image	Note
TS23: lens epithelium	Present		7C
TS23: pigmented retinal epithelium	Present		7C

Structure	Level	Pattern	Image	Note
TS23: trigeminal ganglion	Present	Regionally restricted	7F	Expression in whisker follicle in outer root sheath along the shaft and in the layer surrounding the bulb.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present		8C
TS22: pigmented retinal epithelium	Present		8C
TS22: tongue	Present		8C
TS22: tooth	Present		8C

Structure	Level	Pattern	Image	Note
TS25: cochlea	Present		8G
TS25: olfactory epithelium	Present		8G
TS25: pigmented retinal epithelium	Present		8G