Probe:	mSlit1 probe
Probe preparation:	labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: neural tube floor plate	Present		3A
TS13: neural tube roof plate	Present		3A

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		3B
TS17: neural tube roof plate	Present		3B

Structure	Level	Pattern	Image	Note
TS19: neural tube floor plate	Present		3C
TS19: neural tube roof plate	Present		3C

Structure	Level	Pattern	Image	Note
TS21: spinal cord floor plate	Present		3D
TS21: spinal cord roof plate	Weak		3D
TS21: spinal cord	Weak	Regionally restricted	3D	Weak expression in motor columns and commissural neurons.
TS21: dorsal root ganglion	Weak		3D

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch	Present		4A
TS13: 2nd branchial arch	Present		4A
TS13: neural tube floor plate	Present		4A
TS13: neural tube roof plate	Present		4A

Structure	Level	Pattern	Image	Note
TS14: future brain	Present	Regionally restricted	4B	Expression in neural fold overlying the fourth ventricle and midbrain.
TS14: 1st branchial arch	Present		4B
TS14: 2nd branchial arch	Present		4B
TS14: neural tube floor plate	Present		4B
TS14: neural tube roof plate	Present		4B

Structure	Level	Pattern	Image	Note
TS14: future brain	Present	Regionally restricted	4C	Expression in neural fold overlying the fourth ventricle and midbrain.
TS14: 1st branchial arch	Present		4C
TS14: 2nd branchial arch	Present		4C
TS14: 3rd branchial arch	Present		4C
TS14: neural tube floor plate	Present		4C
TS14: neural tube roof plate	Present		4C

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch	Present		4D
TS15: 2nd branchial arch	Present		4D
TS15: 3rd branchial arch	Present		4D
TS15: neural tube roof plate	Present		4D

Structure	Level	Pattern	Image	Note
TS17: midbrain	Strong	Regionally restricted	5A	Intense expression in developing tectum.
TS17: neural tube roof plate	Present		5A
TS17: somite	Present	Regionally restricted	5A	Segmented pattern of expression in posterior somites.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Strong	Regionally restricted	5B	Intense expression in midline of developing tectum.
TS17: hindbrain	Strong	Regionally restricted	5B	Intense expression in rhombic lip.
TS17: neural tube roof plate	Present		5B
TS17: dermomyotome	Present	Regionally restricted	5B	Expression in a segmented pattern in posterior dermomyotome.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Strong	Regionally restricted	5C	Intense expression in midline of developing tectum.
TS19: cerebellum primordium	Strong		5C	Intense expression in rhombic lip
TS19: neural tube roof plate	Present		5C
TS19: dermomyotome	Present	Regionally restricted	5C	Expression in a segmented pattern in posterior dermomyotome, most prominent caudally.

Structure	Level	Pattern	Image	Note
TS19: nasal pit	Weak		5D	Weak expression in nasal pit.

Structure	Level	Pattern	Image	Note
TS17: dermomyotome	Present	Regionally restricted	6G	Expression in a segmented pattern in posterior dermomyotome.

Structure	Level	Pattern	Image	Note
TS17: dermomyotome	Present	Regionally restricted	6J	Expression in a segmented pattern in posterior dermomyotome.

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Present	Regionally restricted	7A	Expression in neural layer of the retina. Strongest expression in the inner nuclear layer.
TS22: lens epithelium	Weak		7A

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Present		8A
TS22: lateral ganglionic eminence	Present		8A
TS22: medial ganglionic eminence	Present		8A
TS22: neural retinal epithelium	Present	Regionally restricted	8A	Expression in neural layer of the retina, especially in the inner nuclear layer.

Structure	Level	Pattern	Image	Note
TS25: neural retina	Present	Regionally restricted	8E	Expression in neural layer of the retina.
TS25: retina inner nuclear layer	Strong		8E	Strongest expression in the inner nuclear layer.
TS25: trigeminal ganglion	Present		8E
TS25: olfactory epithelium	Weak		8E

Structure	Level	Pattern	Image	Note
TS25: diencephalon	Present	Regionally restricted		Expression in ventral diencephalon, dorsal mesencephalon, and ventral metencephalon.
TS25: metencephalon	Present	Regionally restricted		Expression in ventral diencephalon, dorsal mesencephalon, and ventral metencephalon.
TS25: midbrain	Present	Regionally restricted		Expression in ventral diencephalon, dorsal mesencephalon, and ventral metencephalon.