Probe:	R-cadherin probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	2A	Expression was detected in the ventral thalamus. Expression overlapped with both Pax6 and Dlx2 at their caudal edges. Expression ended at the ventral/dorsal thalamus boundary.
TS20: midbrain	Present	Regionally restricted	2A	Expression was detected in the pretectum. Expression overlapped with Pax6 at the caudal limits.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	3C , 3B , 3D	Expression was detected in the ventral thalamus. Essentially all the cells were positive, at least at the ependymal layer level. The border of the positive cells at the ventral/dorsal thalamus and alar/basal boundary was sharp.
TS19: midbrain	Present	Regionally restricted	3F , 3E , 3B	Expression was detected in the pretectum. Essentially all the cells were positive, at least at the ependymal layer level. The border of positive cells at the pretectum/midbrain boundary was sharp but the rostral end of the pretectum gradually decreased.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	4A	Expression was detected in the ventrocaudal region of the midbrain, in a pair of nucleus-like domains that are presumably future trochlear nuclei.