Gene Expression Data
Assay Details
Assay
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Reference:
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J:39345
Inoue T, et al., Cadherin-6 expression transiently delineates specific rhombomeres, other neural tube subdivisions, and neural crest subpopulations in mouse embryos. Dev Biol. 1997 Mar 15;183(2):183-94
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5490617
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Gene symbol:
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Cdh6
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Gene name:
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cadherin 6
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Probe:
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Cad6 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 7.5
(more )
Specimen
2A:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 7.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo |
Present |
Regionally restricted |
2A
|
Expression is in an anterior crescent area.
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Specimen
2B:
embryonic day 7.75
(more )
Specimen
2B:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 7.75
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2C:
embryonic day 8.0
(more )
Specimen
2C:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2D:
embryonic day 8.0
(more )
Specimen
2D:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2E:
embryonic day 8.0
(more )
Specimen
2E:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: future midbrain |
Present |
Regionally restricted |
2E
|
Expression is at the ridge of the neural fold.
|
TS12: future hindbrain |
Present |
Regionally restricted |
2E
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Expression is at the ridge of the neural fold of the anterior hindbrain.
|
Specimen
2F:
embryonic day 8.0
(more )
Specimen
2F:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2G:
embryonic day 8.5
(more )
Specimen
2G:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2H:
embryonic day 8.5
(more )
Specimen
2H:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2I:
embryonic day 8.5
(more )
Specimen
2I:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2J:
embryonic day 8.5
(more )
Specimen
2J:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3A:
embryonic day 8.0
(more )
Note: Double labeled: blue - Cdh6; red - Egr2 (assay MGI:5490628).
Specimen
3A:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: blue - Cdh6; red - Egr2 (assay MGI:5490628).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: future hindbrain |
Present |
Regionally restricted |
3A
|
The anterior limit of expression is the rhombomere 4/5 boundary and is virtually negative in rhombomere 3 and 5.
|
Specimen
3B:
embryonic day 8.5
(more )
Note: Double labeled: blue - Cdh6; red - Egr2 (assay MGI:5490628).
Specimen
3B:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: blue - Cdh6; red - Egr2 (assay MGI:5490628).
|
Specimen
4A:
embryonic day 9.0
(more )
Specimen
4A:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 9.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4B:
embryonic day 9.5
(more )
Specimen
4B:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4C:
embryonic day 9.5
(more )
Specimen
4C:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
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Fixation:
|
4% Paraformaldehyde
|
Specimen
4D:
embryonic day 12.5
(more )
Note: Dissected brain, the cerebral hemisphere was removed in this sample.
Specimen
4D:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Dissected brain, the cerebral hemisphere was removed in this sample.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hypothalamus ventricular layer |
Present |
|
4D
|
|
TS20: diencephalon ventricular layer |
Weak |
Regionally restricted |
4D
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As a line at the zona limitans intrathalamica along the boundary between the dorsal and ventral thalamus and along the alar/basal boundary in the diencephalon and midbrain.
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TS20: midbrain ventricular layer |
Weak |
Regionally restricted |
4D
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As a line at the zona limitans intrathalamica along the boundary between the dorsal and ventral thalamus and along the alar/basal boundary in the diencephalon and midbrain.
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TS20: thalamus ventricular layer |
Weak |
Regionally restricted |
4D
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As a line at the zona limitans intrathalamica along the boundary between the dorsal and ventral thalamus and along the alar/basal boundary in the diencephalon and midbrain.
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Specimen
4E:
embryonic day 12.5
(more )
Note: Isolated telencephalon.
Specimen
4E:
(close )
Genetic Background:
|
ICR
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Age:
|
embryonic day 12.5
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
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Isolated telencephalon.
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Specimen
5A:
embryonic day 12.0
(more )
Specimen
5A:
(close )
Genetic Background:
|
ICR
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Age:
|
embryonic day 12.0
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Sex:
|
Not Specified
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Type:
|
section from whole mount
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Fixation:
|
4% Paraformaldehyde
|
Specimen
5B, C:
embryonic day 12.0
(more )
Note: Double labeled: blue - Cdh6; brown - Nefm (assay MGI:5490637).
Specimen
5B, C:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 12.0
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Sex:
|
Not Specified
|
Type:
|
section from whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: blue - Cdh6; brown - Nefm (assay MGI:5490637).
|
Specimen
5E:
embryonic day 12.0; Pax3Sp-2H/Pax3 +
(more )
Specimen
5E:
(close )
Genetic Background:
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involves: 101 * C3H/He
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Mutant Allele(s):
|
Pax3Sp-2H/Pax3+
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Age:
|
embryonic day 12.0
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Sex:
|
Not Specified
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Type:
|
section from whole mount
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Fixation:
|
4% Paraformaldehyde
|
Specimen
5F:
(close )
Genetic Background:
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involves: 101 * C3H/He
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Mutant Allele(s):
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Pax3Sp-2H/Pax3Sp-2H
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Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
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Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5G:
embryonic day 12.0
(more )
Specimen
5G:
(close )
Genetic Background:
|
ICR
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Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
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Type:
|
section from whole mount
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Fixation:
|
4% Paraformaldehyde
|
Specimen
5H:
embryonic day 15.0
(more )
Specimen
5H:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 15.0
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Sex:
|
Not Specified
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Type:
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section from whole mount
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Fixation:
|
4% Paraformaldehyde
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Specimen
not shown:
embryonic day 12.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
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Type:
|
section from whole mount
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Fixation:
|
4% Paraformaldehyde
|
Specimen
6B +/-:
embryonic day 8.0; Hoxa1tm1Mrc/Hoxa1 +
(more )
Note: 4 somites.
Specimen
6B +/-:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd
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Mutant Allele(s):
|
Hoxa1tm1Mrc/Hoxa1+
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Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
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4 somites.
|
Specimen
6B -/-:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Hoxa1tm1Mrc/Hoxa1tm1Mrc
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Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
4 somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: future hindbrain |
Present |
Regionally restricted |
6B -/-
|
The anterior limit of expression has retreated compared to wild-type.
|
Specimen
6C:
embryonic day 8.5; Hoxa1tm1Mrc/Hoxa1 +
(more )
Note: 9 somites.
Specimen
6C:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd
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Mutant Allele(s):
|
Hoxa1tm1Mrc/Hoxa1+
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Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
9 somites.
|
Specimen
6D:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd
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Mutant Allele(s):
|
Hoxa1tm1Mrc/Hoxa1tm1Mrc
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
9 somites.
|
Specimen
6E:
embryonic day 8.5; Hoxa1tm1Mrc/Hoxa1 +
(more )
Note: 9 somites.
Specimen
6E:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd
|
Mutant Allele(s):
|
Hoxa1tm1Mrc/Hoxa1+
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
9 somites.
|
Specimen
6F:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd
|
Mutant Allele(s):
|
Hoxa1tm1Mrc/Hoxa1tm1Mrc
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
9 somites.
|
Specimen
6G:
embryonic day 8.5; Hoxa1tm1Mrc/Hoxa1 +
(more )
Note: 11 somites.
Specimen
6G:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd
|
Mutant Allele(s):
|
Hoxa1tm1Mrc/Hoxa1+
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
11 somites.
|
Specimen
6H:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd
|
Mutant Allele(s):
|
Hoxa1tm1Mrc/Hoxa1tm1Mrc
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
11 somites.
|
Specimen
6I:
embryonic day 9.5; Hoxa1tm1Mrc/Hoxa1 +
(more )
Specimen
6I:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd
|
Mutant Allele(s):
|
Hoxa1tm1Mrc/Hoxa1+
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
6J:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd
|
Mutant Allele(s):
|
Hoxa1tm1Mrc/Hoxa1tm1Mrc
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
|