Gene Expression Data
Assay Details
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Assay
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Reference:
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J:14821
Ng LJ, et al., Preferential expression of alternatively spliced mRNAs encoding type II procollagen with a cysteine-rich amino-propeptide in differentiating cartilage and nonchondrogenic tissues during early mouse development. Dev Biol. 1993 Oct;159(2):403-17
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:5490237
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Gene symbol:
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Col2a1
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Gene name:
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collagen, type II, alpha 1
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Probe:
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OLJ12, OLJ8
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Probe preparation:
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labelled with P32 DNA
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Visualized with:
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Autoradiography
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Assay notes:
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Primer set specific for type IIB transcripts. PCR products were verified by filter hybridization using labeled pNJ58 (MGI:9291) as a probe.
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Results
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| Lane not used in this assay |
| P newborn
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TS27: cartilage
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Present
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20 µg; total RNA |
Not Specified |
| Not Specified |
| Lane not used in this assay |
| E9.5
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TS15: heart
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Present
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20 µg; total RNA |
Not Specified |
| Not Specified |
| Lane not used in this assay |
| E11.5
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TS19: heart
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Present
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20 µg; total RNA |
Not Specified |
| Not Specified |
| Lane not used in this assay |
| E14.5
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TS22: brain
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Present
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20 µg; total RNA |
Not Specified |
| Not Specified |
| Lane not used in this assay |
| E15.5
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TS23: brain
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Present
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20 µg; total RNA |
Not Specified |
| Not Specified |
| Lane not used in this assay |
| E16.5
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TS24: brain
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Present
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20 µg; total RNA |
Not Specified |
| Not Specified |
| Lane not used in this assay |
| E17.5
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TS25: brain
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Present
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20 µg; total RNA |
Not Specified |
| Not Specified |
| Lane not used in this assay |
| Control |
| Lane not used in this assay |
| Control |
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Analysis Tools
