Probe:	Esrp1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present	Widespread	4A
TS9: extraembryonic ectoderm	Present	Widespread	4A

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present	Widespread	4B
TS9: extraembryonic ectoderm	Present	Widespread	4B

Structure	Level	Pattern	Image	Note
TS9: extraembryonic visceral endoderm	Absent		4C
TS9: parietal endoderm	Absent		4C

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Ambiguous		4D	Expression was reduced in the primitive streak.
TS11: definitive endoderm	Absent		4D
TS11: extraembryonic visceral endoderm	Absent		4D

Structure	Level	Pattern	Image	Note
TS11: chorion ectoderm	Strong		4E
TS11: embryo	Present	Regionally restricted	4E	Expression was detected in regions underlying the headfolds.
TS11: ectoplacental cone	Absent		4E
TS11: extraembryonic visceral endoderm	Absent		4E

Structure	Level	Pattern	Image	Note
TS11: chorion ectoderm	Strong		4F
TS11: ectoplacental cone	Absent		4F
TS11: extraembryonic visceral endoderm	Absent		4F

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	4G	Expression was detected in the epiblast.
TS11: primitive streak	Absent		4G
TS11: embryo ectoderm	Present	Regionally restricted	4G	Expression was detected at the junction between the neural ectoderm and surface ectoderm.
TS11: definitive endoderm	Present		4G

Structure	Level	Pattern	Image	Note
TS11: neural fold	Present	Regionally restricted	4H	Expression was detected in the open neural folds, in the hinge points where neural fold closures will initiate.
TS11: embryo endoderm	Present	Regionally restricted	4H	Expression was detected in the foregut.
TS11: extraembryonic component	Present	Regionally restricted	4H	Expression was detected in a subset of cells between the epiblast and the growing amnion.
TS11: allantois	Absent		4H
TS11: yolk sac	Absent		4H

Structure	Level	Pattern	Image	Note
TS11: chorion ectoderm	Strong		4I
TS11: neural fold	Present	Regionally restricted	4I	Expression was detected in the open neural folds, in the hinge points where neural fold closures will initiate.
TS11: extraembryonic component	Present	Regionally restricted	4I	Expression was detected in a subset of cells between the epiblast and the growing amnion.
TS11: allantois	Absent		4I
TS11: yolk sac	Absent		4I

Structure	Level	Pattern	Image	Note
TS13: embryo endoderm	Present	Regionally restricted	5A	Expression was detected in the definitive endoderm.
TS13: 1st branchial pouch	Present		5A
TS13: foregut diverticulum endoderm	Present		5A

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch	Present		5B
TS13: 1st branchial pouch	Present		5B

Structure	Level	Pattern	Image	Note
TS13: embryo endoderm	Present		5C

Structure	Level	Pattern	Image	Note
TS13: chorion	Present		5D
TS13: head	Present	Regionally restricted	5D	Expression was detected in the headfolds.

Structure	Level	Pattern	Image	Note
TS15: oral region	Present	Regionally restricted	6A	Expression was detected in the oral epithelium.
TS15: 1st branchial arch ectoderm	Present		6A
TS15: branchial pouch	Present		6A
TS15: embryo endoderm	Present		6A
TS15: otocyst	Present		6A
TS15: heart	Absent		6A

Structure	Level	Pattern	Image	Note
TS15: branchial pouch	Present	Regionally restricted	6B	Expression was restricted to the ventral region of the pharyngeal pouches.

Structure	Level	Pattern	Image	Note
TS15: embryo endoderm	Present		6C

Structure	Level	Pattern	Image	Note
TS15: hindgut	Present		6D
TS15: nephric duct	Present		6D

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present		6E

Structure	Level	Pattern	Image	Note
TS15: oral region	Present	Regionally restricted	6F	Expression was detected in the oral epithelium.
TS15: 1st branchial arch ectoderm	Present		6F
TS15: branchial groove	Present		6F
TS15: branchial pouch	Present		6F
TS15: heart	Absent		6F

Structure	Level	Pattern	Image	Note
TS15: chorioallantoic placenta	Present	Regionally restricted	6G	Expression was detected in the chorion.
TS15: labyrinthine zone	Present	Regionally restricted	6G	Expression was detected in differentiated trophoblast cells surrounding the maternal sinuses in the distal end of labyrinth layer and in a subset of trophoblast cells in the proximal region of the labyrinth layer.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial pouch	Present		7A
TS17: 2nd branchial pouch	Present		7A
TS17: 3rd branchial pouch	Present		7A
TS17: 4th branchial pouch	Present		7A
TS17: nasal pit	Present		7A
TS17: nephric duct	Present		7A
TS17: otocyst	Present		7A
TS17: surface ectoderm	Present	Regionally restricted	7A	Expression was detected in the surface ectoderm of the ventral surface between the outgrowing limb and the body wall.
TS17: heart	Absent		7A

Structure	Level	Pattern	Image	Note
TS17: Rathke's pouch	Present		7B
TS17: surface ectoderm	Present	Regionally restricted	7B	Expression was detected in the nasopharyngeal groove, or surface ectoderm distal to the developing lens.

Structure	Level	Pattern	Image	Note
TS17: nephric duct	Present		7C
TS17: genitourinary system	Present	Regionally restricted	7C	Expression was detected in the ureteric bud.

Structure	Level	Pattern	Image	Note
TS17: nephric duct	Present		7D
TS17: genitourinary system	Present	Regionally restricted	7D	Expression was detected in the urogenital sinus.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial pouch	Present		7E
TS17: 2nd branchial pouch	Present		7E
TS17: 3rd branchial pouch	Present		7E
TS17: 4th branchial pouch	Present		7E
TS17: nasal pit	Present		7E
TS17: otocyst	Present		7E
TS17: heart	Absent		7E

Structure	Level	Pattern	Image	Note
TS17: surface ectoderm	Present	Regionally restricted	7F	Expression was detected in the surface ectoderm of the ventral surface between the outgrowing limb and the body wall.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch ectoderm	Present		7G
TS17: branchial groove ectoderm	Present		7G
TS17: branchial pouch endoderm	Present		7G

Structure	Level	Pattern	Image	Note
TS17: embryo endoderm	Present		7H
TS17: neural tube	Absent		7H
TS17: notochord	Absent		7H

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present	Regionally restricted	7I	Expression was ventral-laterally restricted in the otic vesicle.

Structure	Level	Pattern	Image	Note
TS20: eye	Present		8A
TS20: lens vesicle	Present		8A
TS20: pinna	Present		8A
TS20: vibrissa	Present		8A
TS20: integumental system	Present	Regionally restricted	8A	Expression was detected in the hair follicles.
TS20: face	Present	Regionally restricted	8A	Expression was detected in the sensory placodes of the face.
TS20: mammary bud	Present		8A

Structure	Level	Pattern	Image	Note
TS20: pituitary gland	Present		8B
TS20: tongue	Present	Regionally restricted	8B	Expression was detected in the surface of the tongue.
TS20: liver	Absent		8B
TS20: pancreas	Present		8B , 8F
TS20: primary sex cord	Present		8B , 8F
TS20: primitive collecting duct	Present	Regionally restricted	8F , 8B	Expression was detected in the ureteric branches of the kidney.

Structure	Level	Pattern	Image	Note
TS20: lung	Present	Regionally restricted	8C	Expression was detected in the developing alveoli of the lung.

Structure	Level	Pattern	Image	Note
TS20: oral region gland	Present	Regionally restricted	8D	Expression was detected in the salivary glands.

Structure	Level	Pattern	Image	Note
TS20: intestine	Present	Regionally restricted	8E	Expression was detected in the inner wall of the intestine.