Probe:	A
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent
TS11: extraembryonic component	Absent

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present	Regionally restricted	4a/a'	The anterior boundary of expression is at the level of the third cervical somite.
TS13: lateral plate mesoderm	Present	Regionally restricted	4a/a'	The anterior boundary of expression is at the level of the fifth cervical somite.
TS13: somite	Present	Regionally restricted	4a/a'	The anterior boundary of expression is at the level of the fifth cervical somite.

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present		4b/b'	Expression is throughout the dorsoventral axis of the neural tube.
TS13: lateral plate mesoderm	Present		4b/b'
TS13: somite	Present		4b/b'
TS13: embryo endoderm	Absent		4b/b'
TS13: future hindbrain	Absent		4b/b'
TS13: notochord	Absent		4b/b'

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present	Regionally restricted	4c/c'	Expression is in the posterior neural tube.
TS13: embryo mesenchyme	Present	Regionally restricted	4c/c'	Expression is in posterior mesoderm.
TS13: somite	Present	Regionally restricted	4c/c'	Absent in anterior somites.
TS13: future brain	Absent		4c/c'

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	4e/e'	The anterior boundary of expression is at the level of the third cervical somite.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		4f/f'	Expression is throughout the dorsoventral axis of the neural tube.
TS15: spinal ganglion	Present	Regionally restricted	4f/f'	Expression is in the spinal ganglia posterior to the third cervical somite.
TS15: embryo mesenchyme	Present	Regionally restricted	4f/f'	Expression is posterior to the fifth cervical somite.

Structure	Level	Pattern	Image	Note
TS15: mesonephros mesenchyme	Present	Regionally restricted		Expression is in the mesonephric mesenchyme surrounding the hindgut.
TS15: mesonephric tubule	Absent

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	5a/a'	The anterior boundary of expression is at the level of the first cervical somite.
TS17: spinal ganglion	Present	Regionally restricted	5a/a'	The anterior boundary of expression is at the level of the first cervical somite.
TS17: trunk mesenchyme	Absent		5a/a'

Structure	Level	Pattern	Image	Note
TS17: spinal ganglion	Present	Regionally restricted	5b/b'	The anterior boundary of expression is at the level of the first cervical somite.
TS17: future spinal cord	Present	Graded	5b/b'	Decreasing intensity along the anteroposterior axis.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	5c/c'	Expression is dorsal and is absent in differentiating motor neurons.

Structure	Level	Pattern	Image	Note
TS19: neural tube ventricular layer	Present	Regionally restricted	6a/a'	In the anterior spinal cord the stongly labeled cells are along the outer edge of the ventricular zone. The anterior boundary is at the first cervical somite. In the posterior spinal cord labeling is strongest dorsolaterally.
TS19: spinal ganglion	Weak		6a/a'
TS19: neural tube basal columns	Absent		6a/a'

Structure	Level	Pattern	Image	Note
TS21: alar columns	Present		6b/b'	Expression is in the dorsal horn.

Structure	Level	Pattern	Image	Note
TS27: spinal cord dorsal horn	Present	Scattered	6e/e'	The anterior boundary is at the first cervical somite.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	7a/a'	Expression is throughout the dorsoventral axis of the neural tube.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	7b/b'	Expression is dorsal and is absent in differentiating motor neurons.

Structure	Level	Pattern	Image	Note
TS19: neural tube ventricular layer	Present	Regionally restricted	7c/c'	In the anterior spinal cord the stongly labeled cells are along the outer edge of the ventricular zone.

Structure	Level	Pattern	Image	Note
TS21: alar columns	Present	Regionally restricted	7d/d'	Expression is in the dorsal horn.