Probe:	RZPDp981D0934D
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	1A	Expressed in two bilateral fields under the primitive head fold region and on either side of the notochordal plate.

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	1B	Expressed in two bilateral fields under the primitive head fold region and on either side of the notochordal plate.

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	1C	Crescent-like shape expression under the head folds.

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	1D	Crescent-like shape expression under the head folds.

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present	Regionally restricted	1D'	Expressed in the cardiogenic mesoderm.

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present	Regionally restricted	1D"	Expressed in the cardiogenic mesoderm.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	1E	Crescent shape expression in anterior view.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	1F	Crescent shape expression in anterior view.

Structure	Level	Pattern	Image	Note
TS11: cardiogenic plate	Present		1F'

Structure	Level	Pattern	Image	Note
TS11: intraembryonic coelom	Present		1F"	Expressed in the mesothelial cells that give rise to the intra-embryonic coelomic cavity.
TS11: cardiogenic plate	Present		1F"	Expressed in the cardiogenic mesoderm.

Structure	Level	Pattern	Image	Note
TS11: intraembryonic coelom	Present		1F'"	Expressed in the mesothelial cells that give rise to the intra-embryonic coelomic cavity.

Structure	Level	Pattern	Image	Note
TS12: embryo	Present	Regionally restricted	1G	Crescent-like shape expression under the head folds.

Structure	Level	Pattern	Image	Note
TS12: embryo	Present	Regionally restricted	1H	Crescent-like shape expression under the head folds.

Structure	Level	Pattern	Image	Note
TS12: cardiogenic plate	Present		1H'

Structure	Level	Pattern	Image	Note
TS12: primitive heart tube	Present		1H"	Expressed in the myocardial tissue.
TS12: pericardium	Present		1H"

Structure	Level	Pattern	Image	Note
TS12: pericardio-peritoneal component mesothelium	Present	Regionally restricted	1H'"	Expressed in the left- and right-horn of the coelomic canals.
TS12: pericardium	Present	Regionally restricted	1H'"	Expressed in the cardiogenic mesoderm lining the intra-embryonic coelomic cavity.

Structure	Level	Pattern	Image	Note
TS12: primitive heart tube	Present	Regionally restricted	1I	Mainly expressed in first heart field progenitors. Less intense expression in second heart field progenitors.

Structure	Level	Pattern	Image	Note
TS12: primitive heart tube	Present	Regionally restricted	1J	Mainly expressed in first heart field progenitors. Less intense expression in second heart field progenitors.

Structure	Level	Pattern	Image	Note
TS12: primitive heart tube	Present	Regionally restricted	1J'	Mostly expressed by the first heart field progenitors located at a more ventral position.

Structure	Level	Pattern	Image	Note
TS12: pericardium	Present		1J"
TS12: primitive heart tube	Present	Regionally restricted	1J"	Mainly expressed in first heart field progenitors. Less intense expression in second heart field progenitors.

Structure	Level	Pattern	Image	Note
TS12: pericardium	Present	Regionally restricted	1J'"	Expressed in the cardiogenic mesoderm lining the intra-embryonic coelomic cavity.
TS12: primitive heart tube	Present	Regionally restricted	1J'"	Mainly expressed in first heart field progenitors. Less intense expression in second heart field progenitors.

Structure	Level	Pattern	Image	Note
TS12: pericardial component	Present	Regionally restricted	1K	Delineate the pericardium cavity.

Structure	Level	Pattern	Image	Note
TS12: pericardial component	Present	Regionally restricted	1L	Delineate the pericardium cavity.

Structure	Level	Pattern	Image	Note
TS12: primitive heart tube	Present	Regionally restricted	1L'	Expressed in the heart tube tissue adjacent to the ventral pericardium.

Structure	Level	Pattern	Image	Note
TS12: pericardium	Present	Regionally restricted	1L"	Expressed in the ventral mesothelium of the pericardium.

Structure	Level	Pattern	Image	Note
TS12: pericardio-peritoneal component mesothelium	Present		1L'"

Structure	Level	Pattern	Image	Note
TS12: pericardial component	Present	Regionally restricted	1M	Delineate the pericardium cavity.

Structure	Level	Pattern	Image	Note
TS12: pericardial component	Present	Regionally restricted	1N	Delineate the pericardium cavity.

Structure	Level	Pattern	Image	Note
TS12: primitive heart tube	Present	Regionally restricted	1N'	Expressed in the myocardial tissue adjacent to the ventral pericardium. Mainly expressed in first heart field progenitors. Less intense expression in second heart field progenitors.

Structure	Level	Pattern	Image	Note
TS12: pericardium	Present	Regionally restricted	1N"	Expressed in the ventral mesothelium of the pericardium.
TS12: primitive heart tube	Present	Regionally restricted	1N"	Mainly expressed in first heart field progenitors. Less intense expression in second heart field progenitors.

Structure	Level	Pattern	Image	Note
TS12: pericardio-peritoneal component mesothelium	Present		1N'"
TS12: primitive heart tube	Present	Regionally restricted	1N'"	Mainly expressed in first heart field progenitors. Less intense expression in second heart field progenitors.

Structure	Level	Pattern	Image	Note
TS13: pericardial component	Present	Regionally restricted	2A	Expressed at a ventral and dorsal position to the heart forming a V-shape that fuses at the level of the sinus venosus.

Structure	Level	Pattern	Image	Note
TS13: pericardial component	Present	Regionally restricted	2B	Expressed at a ventral and dorsal position to the heart forming a V-shape that fuses at the level of the sinus venosus.
TS13: head mesenchyme	Present		2B

Structure	Level	Pattern	Image	Note
TS13: pericardium	Present		2B'
TS13: heart	Ambiguous		2B'	Residual expression or nonexistent.

Structure	Level	Pattern	Image	Note
TS13: pericardial component	Present	Regionally restricted	2B"	Expression is continuous with the pericardial cavity and most likely composes the right and left anlagen of the developing proepicardium.
TS13: septum transversum	Present		2B"

Structure	Level	Pattern	Image	Note
TS13: pericardio-peritoneal canal mesothelium	Present	Regionally restricted	2B'"	Expressed in the dorsal-lateral side.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		2C

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		2D

Structure	Level	Pattern	Image	Note
TS13: heart	Present		2D'

Structure	Level	Pattern	Image	Note
TS13: sinus venosus	Present	Widespread	2D"	Wide expression extends laterality from the cells adjacent to the right horn to the cells adjacent to the left horn.
TS13: pericardial component	Present	Regionally restricted	2D"	Expressed in the primitive proepicardium.

Structure	Level	Pattern	Image	Note
TS13: pericardio-peritoneal canal mesothelium	Present	Regionally restricted	2D'"	Expressed in the dorsal-lateral side.

Structure	Level	Pattern	Image	Note
TS14: pericardial component	Present	Regionally restricted	2E	Expression delineates the pericardial cavity.
TS14: embryo	Present		2E

Structure	Level	Pattern	Image	Note
TS14: sinus venosus	Present	Widespread	2E'	Wide expression extends laterality from the cells adjacent to the right horn to the cells adjacent to the left horn.
TS14: pericardial component	Present	Regionally restricted	2E'	Expressed in the proepicardium.

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from somatopleure	Present		2E"

Structure	Level	Pattern	Image	Note
TS14: pericardial component	Present	Regionally restricted	2F	Expression delineates the pericardial cavity.
TS14: embryo	Present		2F

Structure	Level	Pattern	Image	Note
TS14: pericardial component	Present	Regionally restricted	2F'	Expressed in the lateral-dorsal region of the proepicardium.
TS14: head mesenchyme	Present		2F'

Structure	Level	Pattern	Image	Note
TS14: peritoneal component	Present	Regionally restricted	2F"	Expressed in a region posterior to the venous pole of the heart corresponding to a more visceral region of the proepicardium.

Structure	Level	Pattern	Image	Note
TS14: peritoneal component	Present	Regionally restricted	2F'"	Expressed in a region posterior to the venous pole of the heart corresponding to a more visceral region of the proepicardium.

Structure	Level	Pattern	Image	Note
TS15: somite	Present		3A
TS15: pericardial component	Present	Regionally restricted	3A	Expressed in the proepicardium.
TS15: anterior cardinal vein	Present		3A

Structure	Level	Pattern	Image	Note
TS15: anterior cardinal vein	Present		3B
TS15: somite	Present		3B
TS15: pericardial component	Present	Regionally restricted	3B	Expressed in the proepicardium.

Structure	Level	Pattern	Image	Note
TS15: heart	Ambiguous		3B'	Expression is residual or non-existent.

Structure	Level	Pattern	Image	Note
TS15: pericardial component	Strong	Regionally restricted	3B"	Expressed in the heart progenitors of the proepicardium.
TS15: dermomyotome	Strong		3B"
TS15: anterior cardinal vein	Strong	Regionally restricted	3B"	Expressed in the vicinity of the anterior cardinal vein.

Structure	Level	Pattern	Image	Note
TS15: pericardial component	Strong	Regionally restricted	3B'"	Expressed in the heart progenitors of the proepicardium.
TS15: anterior cardinal vein	Strong		3B'"
TS15: dermomyotome	Strong		3B'"

Structure	Level	Pattern	Image	Note
TS15: anterior cardinal vein	Present		3C	Expressed in the vicinity of the anterior cardinal vein.
TS15: pericardial component	Present	Regionally restricted	3C	Expressed in the proepicardium.

Structure	Level	Pattern	Image	Note
TS15: anterior cardinal vein	Present	Regionally restricted	3C'	Expressed in the vicinity of the anterior cardinal vein.

Structure	Level	Pattern	Image	Note
TS15: anterior cardinal vein	Present		3C"
TS15: pericardial component	Strong	Regionally restricted	3C"	Expressed in the heart progenitors of the proepicardium.

Structure	Level	Pattern	Image	Note
TS15: anterior cardinal vein	Present		3C'"
TS15: pericardial component	Strong	Regionally restricted	3C'"	Expressed in the heart progenitors of the proepicardium.

Structure	Level	Pattern	Image	Note
TS17: 2nd branchial groove	Present		3F
TS17: pericardium	Present		3F
TS17: septum transversum	Present		3F
TS17: somite	Present		3F
TS17: 1st branchial arch maxillary component	Present	Regionally restricted	3F	Expressed in the maxilary component containing trigeminal (V) neural crest tissue.

Structure	Level	Pattern	Image	Note
TS17: 2nd branchial groove	Present		3G
TS17: pericardium	Present		3G
TS17: septum transversum	Present		3G
TS17: 1st branchial arch maxillary component	Present	Regionally restricted	3G	Expressed in the maxilary component containing trigeminal (V) neural crest tissue.

Structure	Level	Pattern	Image	Note
TS17: pericardium	Present		3G'
TS17: septum transversum	Present		3G'
TS17: 1st branchial arch maxillary component	Present	Regionally restricted	3G'	Expressed at the maxilary component of the first branchial arch containing trigeminal (V) neural crest tissue.

Structure	Level	Pattern	Image	Note
TS17: pericardium	Present		3G"
TS17: septum transversum	Present	Regionally restricted	3G"	Expression is restricted to a small number of cells.

Structure	Level	Pattern	Image	Note
TS17: pericardium	Present		3G'"
TS17: septum transversum	Present		3G'"

Structure	Level	Pattern	Image	Note
TS17: dermomyotome	Present		3G""