Gene Expression Data
Assay Details
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Assay
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Reference:
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J:90448
Cha KB, et al., WNT5A signaling affects pituitary gland shape. Mech Dev. 2004 Feb;121(2):183-94
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5486040
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Gene symbol:
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Cga
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Gene name:
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glycoprotein hormones, alpha subunit
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Antibody:
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Anti-alpha-GSU
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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The fix was 3.7% paraformaldehyde.
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Results
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Specimen
2A:
embryonic day 12.5
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pituitary gland |
Present |
Regionally restricted |
2A
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Expression was detected in thyrotrope and gonadotrope cell populations in the pituitary.
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Specimen
2B:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Wnt5atm1Amc/Wnt5atm1Amc
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pituitary gland |
Present |
Regionally restricted |
2B
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Expression was unchanged in the mutant compared to wild type.
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Specimen
2C:
embryonic day 18.5
(more )
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: pituitary gland |
Present |
Regionally restricted |
2C
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Expression was detected in thyrotrope and gonadotrope cell populations in the pituitary.
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Specimen
2D:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Wnt5atm1Amc/Wnt5atm1Amc
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: pituitary gland |
Present |
Regionally restricted |
2D
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Expression was unchanged in the mutant compared to wild type.
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Analysis Tools