Gene Expression Data
Assay Details
Assay
|
Reference:
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J:186282
Du J, et al., Different expression patterns of Gli1-3 in mouse embryonic maxillofacial development. Acta Histochem. 2012 Oct;114(6):620-5
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5485253
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Gene symbol:
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Gli1
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Gene name:
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GLI-Kruppel family member GLI1
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Probe:
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Gli1 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1A:
embryonic day 11.0
(more )
Specimen
1A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1B:
embryonic day 12.5
(more )
Specimen
1B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1C:
embryonic day 14.5
(more )
Specimen
1C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1D/G:
embryonic day 11.0
(more )
Specimen
1D/G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS18: face |
Present |
Regionally restricted |
1D
,
1G bottom right
,
1G left
,
1G top right
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Faint expression was detected in the epithelium and strong expression in the mesenchyme of the facial dermis region. Expression was faint in the whisker follicle epithelium, but strong in the surrounding mesenchyme.
|
TS18: oral region |
Present |
Regionally restricted |
1G top right
,
1D
,
1G bottom right
,
1G left
|
Expression was detected in the oral mucosa region. No difference was noted between signals from epithelium and mesenchyme. Expression was faint in the tooth germ, but strong in the surrounding mesenchyme.
|
TS18: 1st branchial arch mandibular component |
Strong |
Regionally restricted |
1G bottom right
,
1D
|
Strong expression was detected in Meckel's cartilage.
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Specimen
1E/H:
embryonic day 12.5
(more )
Specimen
1E/H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: oral region |
Weak |
Regionally restricted |
1H bottom right
,
1E
,
1H left
,
1H top right
|
Only faint signals were detected in both epithelium and mesenchyme around the oral dermis and mucosa.
|
TS20: vibrissa follicle |
Weak |
Regionally restricted |
1H top right
,
1E
|
Faint expression was detected in whisker follicle epithelium.
|
TS20: face mesenchyme |
Strong |
Regionally restricted |
1E
,
1H top right
|
Expression was strong in the mesenchyme surrounding the whisker follicles.
|
TS20: Meckel's cartilage |
Strong |
|
1H bottom right
,
1E
|
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Specimen
1F/I:
embryonic day 14.5
(more )
Specimen
1F/I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: oral region |
Present |
Regionally restricted |
1I left
,
1F
,
1I bottom right
,
1I top right
|
Expression in oral mucosa was stronger in epithelium than in mesenchyme.
|
TS22: face |
Present |
Regionally restricted |
1F
,
1I bottom right
,
1I top right
,
1I left
|
Expression was stronger in mesenchyme than epithelium in facial dermis.
|
TS22: vibrissa follicle |
Weak |
Regionally restricted |
1F
,
1I top right
|
Faint expression was detected in whisker follicle epithelium.
|
TS22: face mesenchyme |
Strong |
Regionally restricted |
1F
,
1I top right
|
Strong expression was detected in the mesenchyme surrounding the whisker follicles.
|
TS22: mandible |
Strong |
Regionally restricted |
1I bottom right
,
1F
|
Strong expression was detected in the mandible ossified from Meckel's cartilage.
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