Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:152827
Crossin KL, et al., Cytotactin and its proteoglycan ligand mark structural and functional boundaries in somatosensory cortex of the early postnatal mouse. Dev Biol. 1989 Dec;136(2):381-92
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5484556
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Gene symbol:
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Tnc
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Gene name:
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tenascin C
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Antibody:
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Anti-Cytotactin
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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The fixative was 2.5% paraformaldehyde. The tissue was flattened and cut using a freezing microtome.
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Results
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Specimen
not shown:
postnatal adult
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Specimen
4A:
postnatal day 7
(more )
Specimen
4A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: barrel cortex |
Present |
Non-Uniform |
4A
|
Expression was detected in the barrel walls but was present at very low levels in the barrel hollows.
|
Specimen
5A:
postnatal day 2.5
(more )
Specimen
5A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 2.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: barrel cortex |
Present |
Non-Uniform |
5A
|
Expression was detected in the walls of the developing barrels. Diffuse staining was seen in the barrel hollows. The differential ratio of expression between the barrel walls and hollows appears to be less than at P7.
|
Specimen
5A':
postnatal day 2.5
(more )
Specimen
5A':
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 2.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: barrel cortex |
Present |
Non-Uniform |
5A'
|
Expression was detected in the walls of the developing barrels. Diffuse staining was seen in the barrel hollows. The differential ratio of expression between the barrel walls and hollows appears to be less than at P7.
|
Specimen
5C:
postnatal day 12
(more )
Specimen
5C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 12
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: barrel cortex |
Present |
Regionally restricted |
5C
|
Expression was detected in the barrel walls with very well defined boundaries but the overall amount appears to be diminishing.
|
Specimen
5C':
postnatal day 12
(more )
Specimen
5C':
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 12
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: barrel cortex |
Present |
Regionally restricted |
5C'
|
Expression was detected in the barrel walls with very well defined boundaries but the overall amount appears to be diminishing.
|
Specimen
6A:
postnatal day 13
(more )
Specimen
6A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 13
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Specimen
not shown:
postnatal day 15
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 15
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
|