Tg(EEF1A1-Ahr*)1Pjvt
Transgene Detail
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| Symbol: |
Tg(EEF1A1-Ahr*)1Pjvt |
| Name: |
transgene insertion 1, Raimo Pohjanvirta |
| MGI ID: |
MGI:5475374 |
| Synonyms: |
INS, Tg(EF1alfa-(INS)Ahr), Tg INS-rat AH receptor |
| Transgene: |
Tg(EEF1A1-Ahr*)1Pjvt Location: unknown
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| Alliance: |
Tg(EEF1A1-Ahr*)1Pjvt page
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(EEF1A1-Ahr*)1Pjvt expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Homolog in Mouse |
Note |
| rat |
Ahr (25690) |
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Mutation details: Mice bearing this transgene express the major, "insertion" variant of the aryl-hydrocarbon receptor (AHR) of Han/Wistar rats, which are exceptionally resistant to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) acute toxicity. In Han/Wistar rats, this aberrant receptor, in which the normal 38 C-terminal amino acids are replaced by seven novel amino acids, is translated from two of the three mis-spliced mRNAs resulting from a point mutation in the first nucleotide of intron 10. The transgene contains the promoter of the human eukaryotic translation elongation factor-1alpha1 (EEF1A1, also called "EF1alpha") followed by a 200 bp intron, a ~90 bp fusion of most of the mouse Ahr leader sequence with the 30-bp UTR of the rat Ahr mRNA, a cDNA encoding the Han/Wistar rat "insertion" AHR variant, the SV40 late polyadenylation signal, and nearly 800 bp of rat Ahr 3' UTR; this entire construct is flanked 5' by a 600 bp and 3' by a 250 bp chicken beta-globin core insulator. It was estimated by real-time quantitative polymerase chain reaction (qPCR) analysis that 30 copies of the transgene are present per haploid genome. Transgene-derived mRNA is present in every one of multiple tissues analyzed by reverse transcription-PCR (RT-PCR) and has been shown to be inducible by orally administered TCDD in the liver, where the rat protein also has been detected by western blot analysis.
(J:147952)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:147952 Pohjanvirta R, Transgenic mouse lines expressing rat AH receptor variants--a new animal model for research on AH receptor function and dioxin toxicity mechanisms. Toxicol Appl Pharmacol. 2009 Apr 15;236(2):166-82 |
| All: |
1 reference(s) |
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