Tg(Mt1-SERPINE1)1Upj
Transgene Detail
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| Symbol: |
Tg(Mt1-SERPINE1)1Upj |
| Name: |
transgene insertion 1, The Upjohn Company |
| MGI ID: |
MGI:5475344 |
| Synonyms: |
PAI-1 |
| Transgene: |
Tg(Mt1-SERPINE1)1Upj Location: unknown
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| Alliance: |
Tg(Mt1-SERPINE1)1Upj page
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(Mt1-SERPINE1)1Upj expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Homolog in Mouse |
Note |
| human |
SERPINE1 (5054) |
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Mutation details: This transgene comprises an 1,800 bp DNA fragment containing the mouse metallothionein 1 promoter followed by a 1,397 bp cDNA encoding human SERPINE1 (serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; also called endothelial cell plasminogen activator inhibitor 1) and the bovine growth homone polyadenylation signal. Whereas SERPINE1 mRNA is elevated in livers of 4 day old transgenic mice, by 28 days it has dropped to control levels. Four day-old transgenic mice have greatly elevated levels of the protein, as measured by enzyme-linked immunosorbent assay (ELISA) using a monoclonal anti-SERPINE1 antibody, in spleen, liver and kidney, and lower, but still markedy elevated levels in heart, brain and lung. The liver protein level has diminished >50% by 7 days and 10-fold by 28 days, but remains ~3.5-fold that of controls. Injection of 28 day old mice with zinc sulfate (ZnSO4) induces expression of the protein in plasma (20-fold increase) and in all tissues analyzed.
(J:194516)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:194516 Erickson LA, et al., Development of venous occlusions in mice transgenic for the plasminogen activator inhibitor-1 gene. Nature. 1990 Jul 5;346(6279):74-6 |
| All: |
1 reference(s) |
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Analysis Tools
