Antibody:	anti-Oct-4
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS19: gonad	Present	Regionally restricted	1A	Expression in undifferentiated genital ridges.
TS19: primordial germ cell of gonad	Present		1A

Structure	Level	Pattern	Image	Note
TS20: ovary	Present	Regionally restricted	1B
TS20: germ cell of ovary	Present		1B

Structure	Level	Pattern	Image	Note
TS21: ovary	Present	Regionally restricted	1C
TS21: germ cell of ovary	Present		1C

Structure	Level	Pattern	Image	Note
TS19: gonad	Present	Regionally restricted	1D	Expression in undifferentiated genital ridges.
TS19: primordial germ cell of gonad	Present		1D

Structure	Level	Pattern	Image	Note
TS20: primary sex cord	Present	Regionally restricted	1E
TS20: germ cell of testis	Present		1E

Structure	Level	Pattern	Image	Note
TS21: primary sex cord	Present	Regionally restricted	1F
TS21: germ cell of testis	Present		1F

Structure	Level	Pattern	Image	Note
TS22: germ cell of ovary	Weak	Regionally restricted	2A	Expression only in some clusters of germ cells. No expression in majority of oocytes.

Structure	Level	Pattern	Image	Note
TS24: germ cell of ovary	Absent		2B	Authors report no expression in entire population of meiotic oocytes.

Structure	Level	Pattern	Image	Note
TS22: germ cell of testis	Present	Regionally restricted	2C	Expression in prospermatogonia.

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present	Regionally restricted	2D	Expression in prospermatogonia.

Structure	Level	Pattern	Image	Note
TS27: primary oocyte	Weak	Regionally restricted	5A	Authors report expression in diplotene/dictyate oocytes. Low levels of expression in the cytoplasm of oocytes.

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Strong	Regionally restricted	5B	High expression in germinal vesicles of primary oocytes; upregulared in oocytes within primordial follicles.

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Strong	Regionally restricted	5C	High expression in growing oocytes within mono- and bi-laminar follicles.

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Strong			High expression in fully-grown oocytes in adult females.

Structure	Level	Pattern	Image	Note
TS27: spermatogonium	Present	Regionally restricted	6A	Expression in type A spermatogonia.

Structure	Level	Pattern	Image	Note
TS28: spermatogonium	Present	Regionally restricted	6B	Expression in type A spermatogonia.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule epithelium	Present	Widespread	7A	Expression in spermatogonia distributed within the seminiferous epithelium.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule epithelium	Present	Regionally restricted	7B	Expression in cells distributed at the periphery of the seminiferous epithelium, suggesting that they represent undifferentiated type A spermatogonia.
TS28: spermatogonium	Present		7B	Expression in cells distributed at the periphery of the seminiferous epithelium, suggesting that they represent undifferentiated type A spermatogonia.
TS28: spermatocyte	Absent		7B	No expression in pachytene spermatocytes.

Structure	Level	Pattern	Image	Note
TS28: spermatogonium	Present	Regionally restricted	7C	Expression only in type A spermatogonia; no expression in type B spermatogonia.
TS28: primary spermatocyte	Absent		7C	No expression in meiotic primary and secondary spermatocytes, or spermatids.
TS28: secondary spermatocyte	Absent		7C	No expression in meiotic primary and secondary spermatocytes, or spermatids.
TS28: spermatid	Absent		7C	No expression in meiotic primary and secondary spermatocytes, or spermatids.