Gene Expression Data
Assay Details
Assay
|
Reference:
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J:81317
Yamashiro T, et al., Expression of Runx1, -2 and -3 during tooth, palate and craniofacial bone development. Mech Dev. 2002 Dec;119 Suppl 1:S107-10
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5475170
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Gene symbol:
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Runx1
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Gene name:
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runt related transcription factor 1
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Probe:
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Runx1 cDNA4
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Probe preparation:
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Antisense labelled with S35 RNA
|
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Results
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Specimen
1A:
embryonic day 13.0
(more )
Specimen
1A:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 13.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
2A:
embryonic day 12.0
(more )
Specimen
2A:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 12.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: oral epithelium |
Present |
Regionally restricted |
2A
|
Expression was detected in dental epithelium on the buccal side.
|
Specimen
2D:
embryonic day 13.0
(more )
Specimen
2D:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 13.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: lower jaw mesenchyme |
Present |
Regionally restricted |
2D
|
Expression was detected in the mesenchyme that extends from the perichondrium of Meckel's cartilage, but not in the mesenchyme expanded from ossified mandibular bone.
|
TS21: lower jaw tooth epithelium |
Present |
Regionally restricted |
2D
|
Expression was detected in dental epithelium on the buccal side.
|
Specimen
2D':
embryonic day 13.0
(more )
Specimen
2D':
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 13.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
2G:
embryonic day 14.0
(more )
Specimen
2G:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 14.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: lower jaw tooth epithelium |
Present |
Regionally restricted |
2G
|
Expression was detected in the buccal aspect of the dental epithelium.
|
TS22: lower jaw mesenchyme |
Present |
Regionally restricted |
2G
|
Expression was detected in the mesenchyme that extends from the perichondrium of Meckel's cartilage, but not in the mesenchyme expanded from ossified mandibular bone.
|
Specimen
2G':
embryonic day 14.0
(more )
Specimen
2G':
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 14.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
2J:
embryonic day 16.0
(more )
Specimen
2J:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 16.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: lower jaw tooth epithelium |
Present |
Regionally restricted |
2J
|
Expression was detected in dental epithelium on the buccal and lingual side.
|
Specimen
2J':
embryonic day 16.0
(more )
Specimen
2J':
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 16.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
2M:
embryonic day 18.0
(more )
Specimen
2M:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
2P:
postnatal day 2
(more )
Specimen
2P:
(close )
Genetic Background:
|
NMRI
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: enamel organ |
Weak |
Regionally restricted |
2P
|
Weak expression was detected in the outer enamel epithelium.
|
TS27: lower jaw molar |
Present |
Regionally restricted |
2P
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Some localized expression was detected in ameloblasts.
|
Specimen
3A:
postnatal day 2
(more )
Specimen
3A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3B:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Runx3tm1Yg/Runx3tm1Yg
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Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
4A:
embryonic day 12.0
(more )
Specimen
4A:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 12.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
4B:
embryonic day 13.0
(more )
Specimen
4B:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 13.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: palatal shelf epithelium |
Moderate |
Regionally restricted |
4B
|
Expression was detected in the palatal shelf epithelium contacting the tongue.
|
Specimen
4C:
embryonic day 14.0
(more )
Specimen
4C:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 14.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: palatal shelf epithelium |
Present |
Regionally restricted |
4C
|
Prior to horizontalization of the shelves, the expression was more intense and localized to the tips of the shelves.
|
Specimen
4D:
embryonic day 16.0
(more )
Specimen
4D:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 16.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: palate epithelium |
Present |
Regionally restricted |
4D
|
After the fusion of the shelves, expression was detected at the fusion site.
|
TS24: palate mesenchyme |
Present |
Regionally restricted |
4D
|
After the fusion of the shelves, expression was detected at the fusion site.
|
TS24: nasal cavity epithelium |
Present |
Regionally restricted |
4D
|
Expression was detected at the site of palatal shelf fusion.
|
TS24: oral epithelium |
Present |
Regionally restricted |
4D
|
Expression was detected at the site of palatal shelf fusion.
|
|