Antibody:	Prdm8 antibody 045R2
Detection system:	Alexa Fluor 568
Assay notes:	It is possible that another antibody (MGI:5474232) was used in this assay.

Structure	Level	Pattern	Image	Note
TS26: retina inner nuclear layer	Present		2A , 2C	Expression mainly in the inner nuclear layer.
TS26: neural retina	Present	Regionally restricted	2A , 2C	Expression in a small number of cells in the ganglion cell layer.
TS26: retina outer nuclear layer	Absent		2A , 2C	Expression in innermost layer of inner nuclear layer.

Structure	Level	Pattern	Image	Note
TS27: retina inner nuclear layer	Present	Regionally restricted	3A , 3C	Expression in innermost layer of inner nuclear layer.
TS27: retina ganglion cell layer	Present	Regionally restricted	3A , 3C	Expression in smaller number of cells in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present		4A , 4C
TS28: retina ganglion cell layer	Absent		4A , 4C

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present		4AA , 4Y
TS28: retina ganglion cell layer	Absent		4Y , 4AA

Structure	Level	Pattern	Image	Note
TS21: spinal cord marginal layer	Present		6M

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	6N , 6P	Expression restricted to ventral spinal cord.
TS21: spinal cord marginal layer	Present	Scattered	6N , 6P	Scattered pattern of expression in the marginal zone.

Structure	Level	Pattern	Image	Note
TS21: olfactory cortex	Present	Regionally restricted	7A	Expression first apparent at E13.5 in piriform cortex.

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Present		7B
TS22: piriform cortex	Present		7B

Structure	Level	Pattern	Image	Note
TS24: hippocampus	Present		7C
TS24: piriform cortex	Present		7C

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Present		7D
TS26: piriform cortex	Present		7D

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex intermediate zone	Present		7E , 7G
TS21: cortical plate	Present		7E , 7G

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Present		7J , 7H
TS22: cortical plate	Present		7J , 7H

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Present		7K , 7M
TS24: cortical plate	Present		7K , 7M

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex intermediate zone	Present		7N , 7P
TS26: cortical plate	Present		7N , 7P

Structure	Level	Pattern	Image	Note
TS28: neocortex layer	Present	Regionally restricted	7Q	Expression in a layer-specific manner.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		7R
TS28: piriform cortex	Present		7R

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		7S
TS28: hippocampus	Present		7S

Structure	Level	Pattern	Image	Note
TS28: cortical layer IV	Strong		7V , 7T	Strongest expression in layer 4.