Summary | Transgene origin | Transgene description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Tg(Mchr1-cre)1Emf
Name:	transgene insertion 1, Eleftheria Maratos-Flier
MGI ID:	MGI:5474277
Synonyms:	Mchr1-cre BAC tg
Transgene:	Tg(Mchr1-cre)1Emf  Location: unknown
Alliance:	Tg(Mchr1-cre)1Emf page

Strain of Origin:

C57BL/6J

Transgene Type:    Transgenic (Recombinase) Mutation:    Insertion   Tg(Mchr1-cre)1Emf expression driven by 1 gene Transgene expression driven by: Organism Driver Gene Note mouse Mchr1 (MGI:2180756)   Mutation details: A cre recombinase sequence (1032 bp) was inserted downstream of the Mchr1 promoter on the RP23-202N16 BAC via homologous recombination/BAC recombineering; this replaced nucleotides 4-41 from exon 1 and was designed to disable transcription/translation of the Mchr1 protein from the BAC Mchr1 locus. An frt-flanked kanamycin selection cassette used during homologous recombination/BAC recombineering was removed by transient FLP plasmid injection. The resulting ~198 kbp Mchr1-cre BAC transgene was injected into C57BL/6J embryos to establish transgenic founder animals. Founder animals were bred to C57BL/6J mice to generate independent founder lines. One founder line was chosen. (J:194588)

Schematic of the Tg(Mchr1-cre)1Emf transgene

Activity:	Tissue activity of this recombinase allele
Driver:	Mchr1 (mouse) Summary of all recombinase alleles driven by Mchr1. Comparative matrix view of recombinase activities Alliance mouse Mchr1 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:194588 Chee MJ, et al., Neurochemical charaterization of neurons expressing melanin-concentrating hormone receptor 1 in the mouse hypothalamus. J Comp Neurol. 2013 Jul;521(10):2208-34
All:	2 reference(s)