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Gene Expression Data
Assay Details
Assay
Reference: J:182840 Cai C, et al., KHDC1B is a novel CPEB binding partner specifically expressed in mouse oocytes and early embryos. Mol Biol Cell. 2010 Sep 15;21(18):3137-48
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5474193
Gene symbol: Khdc1a
Gene name: KH domain containing 1A
Antibody: anti-NDG1
Detection system: Secondary antibody coupled to Cy5
Assay notes: The antibody used recognizes more than one protein, both family members have been annotated.
Results
Specimen 3D preantral-1: postnatal adult (more )
Structure Level Pattern Image Note
TS28: ovary growing follicle Present 3D left Expressed in pre-antral follicles. Expressed in both oocyte and granulosa cells. Expression in the cytoplasmic was shown.
TS28: mesenchymal stroma of ovary Present 3D left
Specimen 3D preantral-2: postnatal adult (more )
Structure Level Pattern Image Note
TS28: oocyte Present 3D middle Expressed in pre-antral follicles. Expression in the nucleus was shown.
Specimen 3D antral: postnatal adult (more )
Structure Level Pattern Image Note
TS28: ovary antral follicle Present 3D right Expressed in both oocyte and granulosa cells. Expression in the cytoplasmic was shown.
Specimen 3Ea: postnatal adult (more )
Note: Germinal vesicle stage oocyte.
Structure Level Pattern Image Note
TS28: primary oocyte Present 3Ea
Specimen 3Ec: postnatal adult (more )
Note: Meiotic M-phase II stage oocyte.
Structure Level Pattern Image Note
TS28: secondary oocyte Present 3Ec
Specimen 3Ed: embryonic day 0.5 (more )
Note: 1-cell stage embryo.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 3Ed
Specimen 3Ee: embryonic day 0.5 (more )
Note: 1-cell stage embryo.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 3Ee
Specimen 3Ef: embryonic day 1.0 (more )
Note: 2-cell stage embryo.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 3Ef
Specimen 3Eg: embryonic day 2.0 (more )
Note: 4-cell stage embryo.
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present 3Eg
Specimen 3Eh: embryonic day 2.5 (more )
Note: 8-cell stage embryo.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 3Eh
Specimen 3Ei: embryonic day 3.0 (more )
Note: 16-cell stage embryo.
Structure Level Pattern Image Note
TS3: compacted morula Present 3Ei
Specimen 3Ej: embryonic day 3.0 (more )
Note: Morula stage embryo.
Structure Level Pattern Image Note
TS4: compacted morula Present 3Ej
Specimen 3Ek: embryonic day 3.5 (more )
Note: Blastocyst stage embryo.
Structure Level Pattern Image Note
TS4: blastocyst Present 3Ek
Specimen 7E: postnatal adult (more )
Note: Germinal vesicle stage oocyte. Double labeled: magenta - Khdc1a and Khdc1b; green - Cpeb1 (assay MGI:5474207).
Structure Level Pattern Image Note
TS28: primary oocyte Present 7E pan-KHDC1 , 7E merge Colocalized with Cpeb1 in the cytoplasm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory