Gene Expression Data
Assay Details
Assay
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Reference:
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J:141034
Zwaenepoel K, et al., Diversity in genomic organisation, developmental regulation and distribution of the murine PR72/B' subunits of protein phosphatase 2A. BMC Genomics. 2008;9:393
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5474027
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Gene symbol:
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Ppp2r3a
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Gene name:
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protein phosphatase 2, regulatory subunit B'', alpha
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Antibody:
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Anti-PR72 Rec
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Two antibodies were described, but the one used in this assay was not detailed. MGI:5473975 may have been used.
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Results
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Specimen
8Ac:
postnatal adult
(more )
Specimen
8Ac:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
8Af:
postnatal adult
(more )
Specimen
8Af:
(close )
Genetic Background:
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Not Specified
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Age:
|
postnatal adult
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Sex:
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Not Specified
|
Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: skeletal muscle |
Strong |
Non-Uniform |
8Af
|
Intensive staining in the muscle fibers was detected in a striated pattern.
|
Specimen
8Ai:
postnatal adult
(more )
Specimen
8Ai:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cardiac muscle tissue |
Present |
Non-Uniform |
8Ai
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Expression was detected in a striated pattern in longitudinally positioned muscle fibers. Cross-sectional muscle fibers did not show staining in any specific structures.
|
Specimen
8Be:
postnatal adult
(more )
Specimen
8Be:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
8Bg:
postnatal adult
(more )
Note: This section was counterstained with iron hematoxylin (blue).
Specimen
8Bg:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This section was counterstained with iron hematoxylin (blue).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: skeletal muscle |
Present |
Non-Uniform |
8Bg
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Expression was detected in the A-band in skeletal muscle longitudinal sections.
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