Gene Expression Data
Assay Details
Assay
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Reference:
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J:123724
Golonzhka O, et al., Expression of COUP-TF-interacting protein 2 (CTIP2) in mouse skin during development and in adulthood. Gene Expr Patterns. 2007 Aug;7(7):754-60
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5474023
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Gene symbol:
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Mki67
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Gene name:
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antigen identified by monoclonal antibody Ki 67
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Antibody:
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anti-Ki67
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Detection system:
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Secondary antibody coupled to Cy2
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Results
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Specimen
2b,c:
embryonic day 10.5
(more )
Note: Double labeled: red - Mki67; green - Bcl11b (Ctip2; assay MGI:5474019).
Specimen
2b,c:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Mki67; green - Bcl11b (Ctip2; assay MGI:5474019).
|
Specimen
2e,f:
embryonic day 12.5
(more )
Note: Double labeled: red - Mki67; green - Bcl11b (Ctip2; assay MGI:5474019).
Specimen
2e,f:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Double labeled: red - Mki67; green - Bcl11b (Ctip2; assay MGI:5474019).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: epidermis |
Present |
Widespread |
2e
,
2f
|
Expression in virtually all cells of the ectoderm.
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TS20: embryo mesenchyme |
Ambiguous |
|
2f
,
2e
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Authors state that most, but not all, cells of mesenchymal origin were negative for staining.
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Specimen
2h,i:
embryonic day 14.5
(more )
Note: Double labeled: red - Mki67; green - Bcl11b (Ctip2; assay MGI:5474019).
Specimen
2h,i:
(close )
Genetic Background:
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CD-1
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Mki67; green - Bcl11b (Ctip2; assay MGI:5474019).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: epidermis |
Present |
Regionally restricted |
2i
,
2h
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Expression in multiple cells in the basal cell layer.
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TS22: dermis |
Present |
Regionally restricted |
2h
,
2i
|
Expression in a smaller fraction of cells of the future dermis.
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Specimen
2k,l:
embryonic day 16.5
(more )
Note: Double labeled: red - Mki67; green - Bcl11b (Ctip2; assay MGI:5474019).
Specimen
2k,l:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Mki67; green - Bcl11b (Ctip2; assay MGI:5474019).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: epidermis |
Present |
Regionally restricted |
2k
,
2l
|
Expression in only a fraction of basal cells.
|
TS24: dermis |
Present |
Regionally restricted |
2k
,
2l
|
Expression in only a fraction of cells of the dermal compartment.
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Specimen
2n,o:
embryonic day 18.5
(more )
Note: Double labeled: red - Mki67; green - Bcl11b (Ctip2; assay MGI:5474019).
Specimen
2n,o:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Mki67; green - Bcl11b (Ctip2; assay MGI:5474019).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: epidermis |
Present |
Regionally restricted |
2o
,
2n
|
Expression in only a few cells in the basal cell layer.
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TS26: hair follicle |
Present |
Regionally restricted |
2o
,
2n
|
Expression in a considerable number of cells in the hair follicle.
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TS26: dermis |
Ambiguous |
|
2o
,
2n
|
Authors state that the dermis was mostly non-proliferative.
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