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Gene Expression Data
Assay Details
Assay
Reference: J:58692 Frank D, et al., A novel pleckstrin homology-related gene family defined by Ipl/Tssc3, TDAG51, and Tih1: tissue-specific expression, chromosomal location, and parental imprinting. Mamm Genome. 1999 Dec;10(12):1150-9
Assay type: RT-PCR
MGI Accession ID: MGI:5473324
Gene symbol: Phlda3
Gene name: pleckstrin homology like domain, family A, member 3
Probe: 5'-4, 3'-5
Probe preparation: labelled with radioactivity
Visualized with: Phosphorimaging
Assay notes: SSCP analysis for quantitation of allelic expression. The radiolabeled products were digested with KpnI.
Results Image: 2B SSCP E9.5; adult
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex Note
Br P adult TS28: brain Present Present Not Specified (C57BL/6J x CAST/Ei)F1 Not Specified
He P adult TS28: heart Present Present Not Specified (C57BL/6J x CAST/Ei)F1 Not Specified
Lu P adult TS28: lung Present Present Not Specified (C57BL/6J x CAST/Ei)F1 Not Specified
Li P adult TS28: liver Present Present Not Specified (C57BL/6J x CAST/Ei)F1 Not Specified
Ki P adult TS28: metanephros Present Present Not Specified (C57BL/6J x CAST/Ei)F1 Not Specified
Mu P adult TS28: skeletal muscle Present Present Not Specified (C57BL/6J x CAST/Ei)F1 Not Specified
Th P adult TS28: thymus Present Present Not Specified (C57BL/6J x CAST/Ei)F1 Not Specified
Sp P adult TS28: spleen Present Present Not Specified (C57BL/6J x CAST/Ei)F1 Not Specified
B No data stored
C No data stored
1 E9.5 TS15: embryo Present Present Not Specified (C57BL/6J x CAST/Ei)F1 Not Specified Heterozygote
2 E9.5 TS15: embryo Not Applicable Present Not Specified (C57BL/6J x CAST/Ei)F1 Not Specified Homozygous control (C57BL/6J)
3 E9.5 TS15: embryo Present Present Not Specified (C57BL/6J x CAST/Ei)F1 Not Specified Heterozygote
genomic No data stored
Notes:
(a) M. m. castaneus band. The allelic bands were of equal intensity.
(b) C57BL/6J band. The allelic bands were of equal intensity.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory