Phlda3 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:58692 Frank D, et al., A novel pleckstrin homology-related gene family defined by Ipl/Tssc3, TDAG51, and Tih1: tissue-specific expression, chromosomal location, and parental imprinting. Mamm Genome. 1999 Dec;10(12):1150-9
Assay type:	RT-PCR
MGI Accession ID:	MGI:5473307
Gene symbol:	Phlda3
Gene name:	pleckstrin homology like domain, family A, member 3

Probe:	5'-4, 3'-5
Probe preparation:	labelled with radioactivity
Visualized with:	Autoradiography
Assay notes:	C57BL/6J has a restriction enzyme site (TaqI) that results in a 97bp band. M. m. castaneus does not have the site and the allele-specific band is 113bp.

Results

Image: 2B RFLP

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Size Not Specified (b)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
1	E9.5	TS15: embryo	Present	Present	Not Specified	(C57BL/6J x CAST/Ei)F1		Not Specified	Heterozygote
2	E9.5	TS15: embryo	Not Applicable	Present	Not Specified	(C57BL/6J x CAST/Ei)F1		Not Specified	Homozygous control (C57BL/6J)
3	E9.5	TS15: embryo	Present	Present	Not Specified	(C57BL/6J x CAST/Ei)F1		Not Specified	Heterozygote
Pl	E14.5	TS28: placenta	Present	Present	Not Specified	(CAST/Ei x C57BL/6J)F1		Not Specified
EM	E14.5	TS22: yolk sac	Present	Present	Not Specified	(CAST/Ei x C57BL/6J)F1		Not Specified
Br	E14.5	TS22: brain	Present	Present	Not Specified	(CAST/Ei x C57BL/6J)F1		Not Specified
He	E14.5	TS22: heart	Present	Present	Not Specified	(CAST/Ei x C57BL/6J)F1		Not Specified
Lu	E14.5	TS22: lung	Present	Present	Not Specified	(CAST/Ei x C57BL/6J)F1		Not Specified
Li	E14.5	TS22: liver	Present	Present	Not Specified	(CAST/Ei x C57BL/6J)F1		Not Specified
Ki	E14.5	TS22: metanephros	Present	Present	Not Specified	(CAST/Ei x C57BL/6J)F1		Not Specified
Mu	E14.5	TS22: skeletal muscle	Present	Present	Not Specified	(CAST/Ei x C57BL/6J)F1		Not Specified
Pl	E14.5	TS28: placenta	Present	Present	Not Specified	(C57BL/6J x CAST/Ei)F1		Not Specified
EM	E14.5	TS22: yolk sac	Present	Present	Not Specified	(C57BL/6J x CAST/Ei)F1		Not Specified
Br	E14.5	TS22: brain	Present	Present	Not Specified	(C57BL/6J x CAST/Ei)F1		Not Specified
He	E14.5	TS22: heart	Present	Present	Not Specified	(C57BL/6J x CAST/Ei)F1		Not Specified
Lu	E14.5	TS22: lung	Present	Present	Not Specified	(C57BL/6J x CAST/Ei)F1		Not Specified
Li	E14.5	TS22: liver	Present	Present	Not Specified	(C57BL/6J x CAST/Ei)F1		Not Specified
Ki	E14.5	TS22: metanephros	Present	Present	Not Specified	(C57BL/6J x CAST/Ei)F1		Not Specified
Mu	E14.5	TS22: skeletal muscle	Present	Present	Not Specified	(C57BL/6J x CAST/Ei)F1		Not Specified

Notes:
(a) M. m. castaneus band. All organs or tissues except the placenta show equal biallelic expression. Overrepresentation of the maternal allele in the placenta was likely due to contamination with maternal decidual cells.
(b) C57BL/6J band. All organs or tissues except the placenta show equal biallelic expression. Overrepresentation of the maternal allele in the placenta was likely due to contamination with maternal decidual cells.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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