Gene Expression Data
Assay Details
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Assay
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Reference:
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J:188133
Xiol J, et al., A Role for Fkbp6 and the Chaperone Machinery in piRNA Amplification and Transposon Silencing. Mol Cell. 2012 Sep 28;47(6):970-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5471982
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Gene symbol:
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Piwil4
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Gene name:
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piwi-like RNA-mediated gene silencing 4
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Antibody:
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anti-Miwi2
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
2J Fkbp6+/- Miwi2:
embryonic day 18.5; Fkbp6tm1Pngr/Fkbp6 +
(more  )
Specimen
2J Fkbp6+/- Miwi2:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Fkbp6tm1Pngr/Fkbp6+
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Age:
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embryonic day 18.5
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Sex:
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Male
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: germ cell of testis |
Present |
Regionally restricted |
2J Fkbp6+/- Miwi2
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Expression in predominantly nuclear-localized.
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Specimen
2J Fkbp6-/- Miwi2:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Fkbp6tm1Pngr/Fkbp6tm1Pngr
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Age:
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embryonic day 18.5
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Sex:
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Male
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: germ cell of testis |
Present |
Regionally restricted |
2J Fkbp6-/- Miwi2
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Authors report a reduced nuclear accumulation in Fkbp6 mutant male germ cells.
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Analysis Tools