Brk1 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:166082 Escobar B, et al., Brick1 is an essential regulator of actin cytoskeleton required for embryonic development and cell transformation. Cancer Res. 2010 Nov 15;70(22):9349-59
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:5471810
Gene symbol:	Brk1
Gene name:	BRICK1, SCAR/WAVE actin-nucleating complex subunit

Reporter:	lacZ
	Direct Detection

Results

Specimen S6 A: embryonic day 6.5; Brk1^{Gt(DC0002)Wtsi}/Brk1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS9: embryo	Present		S6_A

Specimen S6 B Brk1(+/-) left upper: embryonic day 13.5; Brk1^{Gt(DC0002)Wtsi}/Brk1⁺ (more

)
Note: Fixed with glutaraldehyde and paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS21: embryo	Strong	Ubiquitous	S6_B Brk(+/-) left upper

Specimen S6 B Brk1(+/-) left lower: embryonic day 13.5; Brk1^{Gt(DC0002)Wtsi}/Brk1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS21: brain	Present	Regionally restricted	S6_B Brk(+/-) left lower , S6_B Brk(+/-) NE	Expressed in neuroepithelium.
TS21: dorsal root ganglion	Present		S6_B Brk(+/-) left lower , S6_B Brk(+/-) HR
TS21: heart	Present		S6_B Brk(+/-) left lower , S6_B Brk(+/-) HR
TS21: spinal cord	Present		S6_B Brk(+/-) left lower , S6_B Brk(+/-) HR
TS21: blood vessel	Present		S6_B Brk(+/-) BV , S6_B Brk(+/-) left lower	Expressed in the wall of blood vessels among tissues.
TS21: embryo mesenchyme	Present		S6_B Brk(+/-) left lower , S6_B Brk(+/-) BV	Expressed in fibroblasts.

Specimen S7 B Brk(+/-) Brain: postnatal adult; Brk1^{Gt(DC0002)Wtsi}/Brk1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: brain	Strong	Regionally restricted	S7_B Brk(+/-) Brain	Expressed in brain cortex.

Specimen S7 B Brk(+/-) Cryo Brain: postnatal adult; Brk1^{Gt(DC0002)Wtsi}/Brk1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: brain	Strong	Regionally restricted	S7_B Brk(+/-) Cryo Brain	Expressed in brain cortex.

Specimen S7 B Brk(+/-) Intestine: postnatal adult; Brk1^{Gt(DC0002)Wtsi}/Brk1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: intestine	Strong		S7_B Brk(+/-) Intestine

Specimen S7 B Brk(+/-) Cryo Intestine: postnatal adult; Brk1^{Gt(DC0002)Wtsi}/Brk1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: intestine	Strong		S7_B Brk(+/-) Cryo Intestine

Specimen S7 B Brk(+/-) Kidney: postnatal adult; Brk1^{Gt(DC0002)Wtsi}/Brk1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: glomerular tuft	Strong		S7_B Brk(+/-) Kidney
TS28: renal tubule	Strong		S7_B Brk(+/-) Kidney

Specimen S7 B Brk(+/-) Cryo Kidney: postnatal adult; Brk1^{Gt(DC0002)Wtsi}/Brk1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: glomerular tuft	Strong		S7_B Brk(+/-) Cryo Kidney
TS28: renal tubule	Strong		S7_B Brk(+/-) Cryo Kidney

Specimen S7 B Brk(+/-) Spleen: postnatal adult; Brk1^{Gt(DC0002)Wtsi}/Brk1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: spleen red pulp	Strong		S7_B Brk(+/-) Spleen
TS28: spleen white pulp	Absent		S7_B Brk(+/-) Spleen

Specimen S7 B Brk(+/-) Cryo Spleen: postnatal adult; Brk1^{Gt(DC0002)Wtsi}/Brk1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: spleen red pulp	Strong		S7_B Brk(+/-) Cryo Spleen
TS28: spleen white pulp	Absent		S7_B Brk(+/-) Cryo Spleen

Specimen S7 B Brk(+/-) Testis: postnatal adult; Brk1^{Gt(DC0002)Wtsi}/Brk1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: testis	Present		S7_B Brk(+/-) Testis

Specimen S7 B Brk(+/-) Cryo Testis: postnatal adult; Brk1^{Gt(DC0002)Wtsi}/Brk1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: testis	Present		S7_B Brk(+/-) Cryo Testis

Specimen S7 B Brk(+/-) Lung: postnatal adult; Brk1^{Gt(DC0002)Wtsi}/Brk1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: lung alveolus	Strong		S7_B Brk(+/-) Lung	Expressed in the pneumocytes type I and II cells.
TS28: bronchiole epithelium	Strong		S7_B Brk(+/-) Lung	Expressed in Clara cells.

Specimen S7 B Brk(+/-) Cryo Lung: postnatal adult; Brk1^{Gt(DC0002)Wtsi}/Brk1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: lung alveolus	Strong		S7_B Brk(+/-) Cryo Lung	Expressed in the pneumocytes type I and II cells.
TS28: bronchiole epithelium	Strong		S7_B Brk(+/-) Cryo Lung	Expressed in Clara cells.

Specimen not shown: postnatal adult; Brk1^{Gt(DC0002)Wtsi}/Brk1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	Strong
TS28: cartilage	Strong
TS28: hippocampus	Strong
TS28: skeletal muscle	Strong

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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