Antibody:	Anti-Opa3 (15638-1-AP)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS24: extrinsic ocular muscle	Present		3
TS24: lens anterior epithelium	Present		3
TS24: retina	Present		3
TS24: lens fiber	Present	Regionally restricted	3	Expression was detected in the cortical lens fibers.

Structure	Level	Pattern	Image	Note
TS22: optic nerve	Strong		3_inset	Dense staining was detected in ganglion cell axons as they entered the optic stalk, forming the optic nerve.

Structure	Level	Pattern	Image	Note
TS28: retina layer	Strong	Widespread	4A	Most layers of the retina demonstrated abundant immunostaining.
TS28: retina ganglion cell layer	Strong		4A
TS28: retina inner nuclear layer	Strong		4A
TS28: photoreceptor layer	Strong	Regionally restricted	4A	Expression was detected in the layer containing the cell bodies of the rods and cones.
TS28: photoreceptor layer inner segment	Moderate		4A
TS28: retina inner plexiform layer	Moderate		4A
TS28: retina outer plexiform layer	Moderate		4A

Structure	Level	Pattern	Image	Note
TS28: retina layer	Strong	Widespread	4A inset	Most layers of the retina demonstrated abundant immunostaining.
TS28: retina ganglion cell layer	Strong		4A inset
TS28: retina inner nuclear layer	Strong		4A inset
TS28: photoreceptor layer	Strong	Regionally restricted	4A inset	Expression was detected in the layer containing the cell bodies of the rods and cones.
TS28: photoreceptor layer inner segment	Moderate		4A inset
TS28: retina inner plexiform layer	Moderate		4A inset
TS28: retina outer plexiform layer	Moderate		4A inset

Structure	Level	Pattern	Image	Note
TS28: extrinsic ocular muscle	Strong		4B

Structure	Level	Pattern	Image	Note
TS28: cornea endothelium	Present		4C
TS28: cornea epithelium	Present		4C
TS28: cornea stroma	Absent		4C

Structure	Level	Pattern	Image	Note
TS28: lens epithelium	Present	Regionally restricted	4D	Expression was abundant in the cuboidal anterior lens epithelial cells and in the cortical region of the lens.
TS28: lens fiber	Present	Regionally restricted	4D	Expression was detected in the lens fiber cells beneath the anterior epithelium but was absent from a band of cortical secondary fiber cells.

Structure	Level	Pattern	Image	Note
TS28: lens epithelium	Present	Regionally restricted	4E	Expression was abundant in the cuboidal anterior lens epithelial cells and in the cortical region of the lens.
TS28: lens fiber	Present	Regionally restricted	4E	Expression was detected in the lens fiber cells beneath the anterior epithelium but was absent from a band of cortical secondary fiber cells. The nuclear fibers and the organelle-free zone at the center of the lens had no staining.