Msi1tm1.1(cre/ERT2)Njen
Targeted Allele Detail
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| Symbol: |
Msi1tm1.1(cre/ERT2)Njen |
| Name: |
musashi RNA-binding protein 1; targeted mutation 1.1, Nancy A Jenkins |
| MGI ID: |
MGI:5471313 |
| Synonyms: |
Msi1-CreERT2 |
| Gene: |
Msi1 Location: Chr5:115567734-115593757 bp, + strand Genetic Position: Chr5, 56.09 cM, cytoband E3-F
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| Alliance: |
Msi1tm1.1(cre/ERT2)Njen page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:194120
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| Parent Cell Line: |
Other (see notes) (ES Cell)
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| Strain of Origin: |
(C57BL/6 x 129)F1
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| Allele Type: |
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Targeted (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutations: |
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Insertion, Intragenic deletion
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Msi1tm1.1(cre/ERT2)Njen expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: Sequences of the first exon downstream of the initial ATG of Msi1 in a BAC clone (RP23-268H8) were replaced with a cre/ERT2 cassette, poly A signal and Frt-flanked PGK-neo, by recombineering in bacterial cells. The targeting construct was transfected into B6/129 F1 ES cells. Targeted ES clones were injected into C57BL/6 blastocysts to generate chimeras. Germline mutants were bred to FLPe mice to remove the neo selection cassette. RT-PCR demonstrated that no transcript containing exon 1 is produced from the mutated allele.
(J:194120)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Msi1
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Msi1 Mutation: |
31 strains or lines available
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| Original: |
J:194120 Takeda H, et al., Musashi1-CreER(T2) : A new cre line for conditional mutagenesis in neural stem cells. Genesis. 2013 Feb;51(2):128-34 |
| All: |
4 reference(s) |
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Analysis Tools