Probe:	IMAGE clone 3711771
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS13: migrating neural crest	Absent

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Absent

Structure	Level	Pattern	Image	Note
TS17: migrating neural crest	Absent

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Regionally restricted	2D	Expression was detected along the neuraxis, mainly in the subventricular areas and differentiating fields.
TS19: olfactory epithelium	Strong		2D

Structure	Level	Pattern	Image	Note
TS19: cranial ganglion	Present
TS19: dorsal root ganglion	Present
TS19: inner ear	Present
TS19: head	Present	Uniform		Homogeneous expression was detected in both epithelium and mesenchyme of the orofacial structures.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	2N	Expression was detected in the developing neuraxis, the highest amounts were found in the subventricular zone and differentiating fields.
TS20: cranial ganglion	Present		2N
TS20: inner ear	Present		2N
TS20: pituitary gland	Present		2N

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present		2T

Structure	Level	Pattern	Image	Note
TS20: retina	Present

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Widespread	3D/D'	Expression was detected throughout the neuraxis, particularly in differentiating fields.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Widespread	3I/I'	Expression was detected throughout the neuraxis, particularly in differentiating fields.

Structure	Level	Pattern	Image	Note
TS21: brain	Present		4D

Structure	Level	Pattern	Image	Note
TS21: brain	Present		4I

Structure	Level	Pattern	Image	Note
TS21: cranial ganglion	Present
TS21: dorsal root ganglion	Present

Structure	Level	Pattern	Image	Note
TS22: brain	Present		4N

Structure	Level	Pattern	Image	Note
TS22: cranial ganglion	Present
TS22: dorsal root ganglion	Present

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present		4X
TS26: cochlear duct	Present	Regionally restricted	4X	Expression was detected in the stria vascularis.

Structure	Level	Pattern	Image	Note
TS26: retina	Present	Regionally restricted		Expression was detected in the retinal ganglion cells.
TS26: brain	Present
TS26: tooth epithelium	Trace
TS26: tooth mesenchyme	Trace
TS26: facial bone	Trace
TS26: cartilage	Trace			Basal expression levels were detected in cartilage.
TS26: head mesenchyme	Trace	Regionally restricted		Basal expression levels were detected in the mesenchyme of orofacial structures.

Structure	Level	Pattern	Image	Note
TS19: jaw primordium mesenchyme	Trace	Regionally restricted		Expression was detected in tooth primordia at slightly above background levels in dental epithelium and mesenchyme.
TS19: oral epithelium	Trace	Regionally restricted		Expression was detected in tooth primordia at slightly above background levels in dental epithelium and mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: tooth epithelium	Trace		5H
TS22: tooth mesenchyme	Trace		5H

Structure	Level	Pattern	Image	Note
TS22: skin	Trace

Structure	Level	Pattern	Image	Note
TS20: jaw primordium mesenchyme	Trace	Regionally restricted	6I	Expression was detected at slightly above background levels in the dental epithelium and mesenchyme.
TS20: oral epithelium	Trace	Regionally restricted	6I	Expression was detected at slightly above background levels in the dental epithelium and mesenchyme.
TS20: head	Present	Regionally restricted	6I	Expression was homogeneous in epithelia and mesenchyme of orofacial structures.
TS20: head mesenchyme	Present	Regionally restricted	6I	Expression was homogeneous in epithelia and mesenchyme of orofacial structures.
TS20: palatal shelf	Present		6I

Structure	Level	Pattern	Image	Note
TS21: tooth epithelium	Trace		6N
TS21: tooth mesenchyme	Trace		6N
TS21: cartilage	Present	Regionally restricted	6N	Expression was detected in orofacial structures at levels grossly similar to E12.5.
TS21: facial bone primordium	Present	Regionally restricted	6N	Expression was detected in orofacial structures at levels grossly similar to E12.5.
TS21: tongue	Present	Regionally restricted	6N	Expression was detected in orofacial structures at levels grossly similar to E12.5.
TS21: palatal shelf epithelium	Present	Non-Uniform	6N	The anterior and middle portions of the palate showed higher levels than the posterior segment.
TS21: palatal shelf mesenchyme	Present	Non-Uniform	6N	The anterior and middle portions of the palate showed higher levels than the posterior segment.

Structure	Level	Pattern	Image	Note
TS22: head	Present	Regionally restricted	6W	Expression was ubiquitous in epithelia and mesenchyme of orofacial structures.
TS22: head mesenchyme	Present	Regionally restricted	6W	Expression was ubiquitous in epithelia and mesenchyme of orofacial structures.
TS22: facial bone primordium	Weak	Regionally restricted	6W	Low levels were noted in osteoblasts and chondrocytes.
TS22: palatal shelf mesenchyme	Moderate		6W
TS22: salivary gland epithelium	Trace		6W
TS22: salivary gland mesenchyme	Trace		6W