Probe:	MGC clone 67606
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS13: migrating neural crest	Absent

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Absent

Structure	Level	Pattern	Image	Note
TS17: migrating neural crest	Absent

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Regionally restricted	2C	Expression was ubiquitous in the developing neuraxis. Highest levels of expression were detected in the subventricular zone and differentiating fields.
TS19: olfactory epithelium	Present	Regionally restricted	2C

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Non-Uniform	2I	Expression was ubiquitous in the developing neuraxis. Highest levels of expression were detected in the subventricular zone and differentiating fields.
TS19: future spinal cord	Present	Regionally restricted	2I	Expression was strongest in the motor neuron area.
TS19: blood vessel	Present		2I
TS19: inner ear	Present		2I

Structure	Level	Pattern	Image	Note
TS19: cranial ganglion	Present
TS19: future pituitary	Present
TS19: head	Present	Uniform		Homogeneous expression was detected in both epithelium and mesenchyme of the orofacial structures.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Non-Uniform	2M	Expression was ubiquitous along the neuraxis with the highest amounts being evident in the ventricular and subventricular zone, differentiating fields and the median eminence. Expression was also detected in the meningeal precursors.
TS20: choroid plexus	Present		2M
TS20: medial ganglionic eminence	Present		2M

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	2S	Expression was strong in the motor neuron domain and the ventral neuroepithelium. Expression was also detected in the meningeal precursors.

Structure	Level	Pattern	Image	Note
TS20: pons	Present	Regionally restricted		Expression was detected in the ventricular and subventricular zones, and in the pontine differentiating fields.
TS20: cranial ganglion	Present
TS20: hyaloid vascular plexus	Present
TS20: inner ear	Present
TS20: pituitary gland	Present
TS20: retina	Weak

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Widespread	3C/C'	Expression was detected throughout the neuraxis, particularly in differentiating fields.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Widespread	3H/H'	Expression was detected throughout the neuraxis, particularly in differentiating fields.

Structure	Level	Pattern	Image	Note
TS21: brain	Present	Regionally restricted	4C	Expression was detected in the brain. High levels were detected in the median eminence, subventricular zone and differentiating fields.
TS21: brain meninges	Moderate		4C
TS21: medial ganglionic eminence	Strong		4C

Structure	Level	Pattern	Image	Note
TS21: brain	Present	Regionally restricted	4H	Expression was detected in the brain. High levels were detected in the median eminence, subventricular zone and differentiating fields.
TS21: brain meninges	Moderate		4H
TS21: cranial ganglion	Present		4H
TS21: medial ganglionic eminence	Strong		4H

Structure	Level	Pattern	Image	Note
TS21: choroid plexus	Moderate
TS21: cochlea	Present
TS21: pituitary gland	Present

Structure	Level	Pattern	Image	Note
TS22: brain	Present	Regionally restricted	4M	Expression was detected in the brain. High levels were detected in the median eminence, subventricular zone and differentiating fields.
TS22: brain meninges	Present		4M	Expression was increased compared to E13.5.
TS22: medial ganglionic eminence	Strong		4M

Structure	Level	Pattern	Image	Note
TS22: choroid plexus	Present			Expression was increased compared to E13.5.

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	4R	Expression was increased in the retinal ganglion cell layer at E14.5.
TS22: hyaloid vascular plexus	Present		4R

Structure	Level	Pattern	Image	Note
TS26: brain meninges	Present		4W	Expression was detected in the leptomeninges.
TS26: cochlear duct	Present	Regionally restricted	4W	Expression was detected in the vestibular membrane of Reissner and the stria vascularis.
TS26: inner ear epithelium	Present	Regionally restricted	4W	Expression was detected in the scalae vestibuli and tympani and in their adjacent mesenchyme.
TS26: inner ear mesenchyme	Present	Regionally restricted	4W	Expression was detected in the scalae vestibuli and tympani and in their adjacent mesenchyme.

Structure	Level	Pattern	Image	Note
TS26: brain	Present			Expression was detected, especially in differentiating fields.
TS26: main olfactory bulb	Present			Expression was detected, especially in differentiating fields.
TS26: retina	Present	Regionally restricted		Expression was detected in retinal ganglion cells.
TS26: choroid plexus	Present
TS26: pituitary gland	Present
TS26: dermis	Present
TS26: epidermis	Present
TS26: nasal cavity respiratory epithelium	Weak
TS26: nose	Present	Regionally restricted		Expression was high in the loose connective tissue separating the epithelia from the nasal cavity cartilage.

Structure	Level	Pattern	Image	Note
TS19: jaw primordium mesenchyme	Trace	Regionally restricted		Expression was detected in tooth primordia at slightly above background levels in dental epithelium and mesenchyme.
TS19: oral epithelium	Trace	Regionally restricted		Expression was detected in tooth primordia at slightly above background levels in dental epithelium and mesenchyme.

Structure	Level	Pattern	Image	Note
TS26: incisor	Present	Regionally restricted	5K	Expression was detected in preameloblasts and differentiating ameloblasts, as well as in preodontoblasts and differentiating odontoblasts. Expression declined in differentiated odontoblasts.
TS26: facial bone	Present	Regionally restricted	5K	Expression was high in hypertrophic chondrocytes, perichondrium and osteoblasts in areas undergoing endochondral and intramembranous ossification.

Structure	Level	Pattern	Image	Note
TS26: molar mesenchyme	Present	Regionally restricted	5O	Expression was detected in nascent cusps.
TS26: facial bone	Present	Regionally restricted	5O	Expression was high in hypertrophic chondrocytes, perichondrium and osteoblasts in areas undergoing endochondral and intramembranous ossification.

Structure	Level	Pattern	Image	Note
TS28: viscerocranium	Present	Regionally restricted	5T	Expression was high in hypertrophic chondrocytes, perichondrium and osteoblasts in areas undergoing endochondral and intramembranous ossification.`

Structure	Level	Pattern	Image	Note
TS28: incisor dental papilla	Present		5U
TS28: periodontal ligament	Present		5U
TS28: incisor	Present		5U	Expression was detected in odontoblasts but were more abundant in preodontoblasts. The ameloblastic lineage also showed expression.

Structure	Level	Pattern	Image	Note
TS28: molar dental papilla	Present		5Y , 5Z
TS28: periodontal ligament	Present		5Y , 5Z
TS28: molar	Present		5Z , 5Y	Expression was detected in odontoblasts but were more abundant in preodontoblasts. The ameloblastic lineage also showed expression.
TS28: viscerocranium	Present	Regionally restricted	5Y , 5Z	Expression was high in hypertrophic chondrocytes, perichondrium and osteoblasts in areas undergoing endochondral and intramembranous ossification.

Structure	Level	Pattern	Image	Note
TS20: head	Present	Regionally restricted	6H	Expression was detected ubiquitously in all epithelial and mesenchymal cells of orofacial structures with more abundance in osteoblasts.
TS20: head mesenchyme	Present	Regionally restricted	6H	Expression was detected ubiquitously in all epithelial and mesenchymal cells of orofacial structures with more abundance in osteoblasts.
TS20: palatal shelf	Present		6H

Structure	Level	Pattern	Image	Note
TS20: jaw primordium mesenchyme	Trace	Regionally restricted		Expression was detected at slightly above background levels in the dental epithelium and mesenchyme.
TS20: oral epithelium	Trace	Regionally restricted		Expression was detected at slightly above background levels in the dental epithelium and mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: cartilage	Present	Regionally restricted	6M	Expression was detected in orofacial structures at levels grossly similar to E12.5.
TS21: facial bone primordium	Present	Regionally restricted	6M	Expression was detected in orofacial structures at levels grossly similar to E12.5.
TS21: tongue	Present	Regionally restricted	6M	Expression was detected in orofacial structures at levels grossly similar to E12.5.
TS21: palatal shelf epithelium	Present		6M
TS21: palatal shelf mesenchyme	Present		6M

Structure	Level	Pattern	Image	Note
TS21: tooth epithelium	Trace
TS21: tooth mesenchyme	Trace

Structure	Level	Pattern	Image	Note
TS22: tooth mesenchyme	Present		6V
TS22: head	Present	Regionally restricted	6V	Expression was ubiquitous in epithelia and mesenchyme of orofacial structures.
TS22: head mesenchyme	Present	Regionally restricted	6V	Expression was ubiquitous in epithelia and mesenchyme of orofacial structures.
TS22: facial bone primordium	Present	Regionally restricted	6V	Expression was detected in areas of intramembranous and endochondral ossification in osteoblasts, hypertrophic chondrocytes and perichondrium.
TS22: autonomic ganglion	Present	Regionally restricted	6V	Expression was prominent in the submandibular ganglion.

Structure	Level	Pattern	Image	Note
TS22: salivary gland epithelium	Trace
TS22: salivary gland mesenchyme	Trace

Structure	Level	Pattern	Image	Note
TS23: facial bone primordium	Present	Regionally restricted	6Z'	Expression was detected in areas of intramembranous and endochondral ossification in osteoblasts, hypertrophic chondrocytes and perichondrium.

Structure	Level	Pattern	Image	Note
TS28: nose	Present	Regionally restricted		Expression was high in the loose connective tissue separating the epithelia from the nasal cavity cartilage.
TS28: nasal cavity epithelium	Weak