Probe:	En1 probe4
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS12: future brain	Present	Regionally restricted	1a right	Expression is present in a band in the neural folds at the level of the foregut pocket.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Non-Uniform	2a right	Expression is diminished in the germinal zones of the caudal and dorsal regions where the cerebellum, pons, and colliculi later develop; it is stronger in the differentiated cells.
TS20: midbrain-hindbrain junction	Present	Non-Uniform	2a right	Expression is diminished in the germinal zones of the caudal and dorsal regions where the cerebellum, pons, and colliculi later develop; it is stronger in the differentiated cells.
TS20: hindbrain	Present	Regionally restricted	2a right	Expression is diminished in the germinal zones where the cerebellum and pons originate from; it is stronger in the differentiated cells. It is also present in two stripes that extend from the rostral hindbrain into the spinal cord.
TS20: cerebellum primordium	Present		2a right
TS20: pons	Present		2a right
TS20: spinal cord	Present	Regionally restricted	2a right	Expression is present in two ventrolateral stripes extending the length of the spinal cord; this expression is found in the germinal zone and in differentiated cells.
TS20: dermomyotome	Present		2a right	Expression is present in a swatch of dermomyotome-derived cells extending from the top of the head to the tip of the tail on both sides of the embryo.
TS20: paraxial mesenchyme	Present		2a right	Expression is present in a swatch of dermomyotome-derived cells extending from the top of the head to the tip of the tail on both sides of the embryo.
TS20: tail dermomyotome	Present		2a right	Expression is present in a swatch of dermomyotome-derived cells extending from the top of the head to the tip of the tail on both sides of the embryo.
TS20: tail	Present		2a right

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Non-Uniform	2b right	Expression is diminished in the germinal zones of the caudal and dorsal regions where the cerebellum, pons, and colliculi later develop; it is stronger in the differentiated cells.
TS20: midbrain-hindbrain junction	Present	Non-Uniform	2b right	Expression is diminished in the germinal zones of the caudal and dorsal regions where the cerebellum, pons, and colliculi later develop; it is stronger in the differentiated cells.
TS20: hindbrain	Present	Regionally restricted	2b right	Expression is diminished in the germinal zones where the cerebellum and pons originate from; it is stronger in the differentiated cells. It is also present in two stripes that extend from the rostral hindbrain into the spinal cord.
TS20: cerebellum primordium	Present		2b right
TS20: pons	Present		2b right
TS20: spinal cord	Present	Regionally restricted	2b right	Expression is present in two ventrolateral stripes extending the length of the spinal cord; this expression is found in the germinal zone and in differentiated cells.
TS20: thoracic vertebral cartilage condensation	Present		2b right	Expression is present in the cells of the pericordal tube, which gives rise to the vertebrae; it was not present in the intervertebral disks.
TS20: vertebral pre-cartilage condensation	Present		2b right	Expression is present in the cells of the pericordal tube, which gives rise to the vertebrae; it was not present in the intervertebral disks.
TS20: tail	Present		2b right

Structure	Level	Pattern	Image	Note
TS20: thoracic vertebral cartilage condensation	Present		2c left	Expression is present in the cells of the pericordal tube, which gives rise to the vertebrae; it was not present in the intervertebral disks.
TS20: vertebral pre-cartilage condensation	Present		2c left	Expression is present in the cells of the pericordal tube, which gives rise to the vertebrae; it was not present in the intervertebral disks.
TS20: spinal cord	Present		2c left

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Non-Uniform	3b	Expression is diminished in the germinal zones of the caudal and dorsal regions where the cerebellum, pons, and colliculi later develop; it is stronger in the differentiated cells.
TS20: midbrain-hindbrain junction	Present	Non-Uniform	3b	Expression is diminished in the germinal zones of the caudal and dorsal regions where the cerebellum, pons, and colliculi later develop; it is stronger in the differentiated cells.
TS20: hindbrain	Present	Regionally restricted	3b	Expression is diminished in the germinal zones where the cerebellum and pons originate from; it is stronger in the differentiated cells. It is also present in two stripes that extend from the rostral hindbrain into the spinal cord.
TS20: cerebellum primordium	Present		3b
TS20: pons	Present		3b
TS20: spinal cord	Present	Regionally restricted	3b	Expression is present in two ventrolateral stripes extending the length of the spinal cord; this expression is found in the germinal zone and in differentiated cells.
TS20: dermomyotome	Present	Regionally restricted	3b	Expression is present in a swatch of dermomyotome-derived cells extending from the top of the head to the tip of the tail on both sides of the embryo.
TS20: paraxial mesenchyme	Present	Regionally restricted	3b	Expression is present in a swatch of dermomyotome-derived cells extending from the top of the head to the tip of the tail on both sides of the embryo.
TS20: tail dermomyotome	Present	Regionally restricted	3b	Expression is present in a swatch of dermomyotome-derived cells extending from the top of the head to the tip of the tail on both sides of the embryo.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Non-Uniform	3c middle	Expression is diminished in the germinal zones of the caudal and dorsal regions where the cerebellum, pons, and colliculi later develop; it is stronger in the differentiated cells.
TS20: midbrain-hindbrain junction	Present	Non-Uniform	3c middle	Expression is diminished in the germinal zones of the caudal and dorsal regions where the cerebellum, pons, and colliculi later develop; it is stronger in the differentiated cells.
TS20: hindbrain	Present	Regionally restricted	3c middle	Expression is diminished in the germinal zones where the cerebellum and pons originate from; it is stronger in the differentiated cells. It is also present in two stripes that extend from the rostral hindbrain into the spinal cord.
TS20: cerebellum primordium	Present		3c middle
TS20: pons	Present		3c middle
TS20: spinal cord	Present	Regionally restricted	3c middle	Expression is present in two ventrolateral stripes extending the length of the spinal cord; this expression is found in the germinal zone and in differentiated cells.
TS20: dermomyotome	Present	Regionally restricted	3c middle	Expression is present in a swatch of dermomyotome-derived cells extending from the top of the head to the tip of the tail on both sides of the embryo.
TS20: paraxial mesenchyme	Present	Regionally restricted	3c middle	Expression is present in a swatch of dermomyotome-derived cells extending from the top of the head to the tip of the tail on both sides of the embryo.
TS20: tail dermomyotome	Present	Regionally restricted	3c middle	Expression is present in a swatch of dermomyotome-derived cells extending from the top of the head to the tip of the tail on both sides of the embryo.
TS20: limb	Present	Regionally restricted	3c middle

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Non-Uniform	3d right	Expression is diminished in the germinal zones of the caudal and dorsal regions where the cerebellum, pons, and colliculi later develop; it is stronger in the differentiated cells.
TS20: midbrain-hindbrain junction	Present	Non-Uniform	3d right	Expression is diminished in the germinal zones of the caudal and dorsal regions where the cerebellum, pons, and colliculi later develop; it is stronger in the differentiated cells.
TS20: hindbrain	Present	Regionally restricted	3d right	Expression is diminished in the germinal zones where the cerebellum and pons originate from; it is stronger in the differentiated cells. It is also present in two stripes that extend from the rostral hindbrain into the spinal cord.
TS20: cerebellum primordium	Present		3d right
TS20: pons	Present		3d right
TS20: spinal cord	Present	Regionally restricted	3d right	Expression is present in two ventrolateral stripes extending the length of the spinal cord; this expression is found in the germinal zone and in differentiated cells. It is not present in the cells nearest the lumen.
TS20: dermomyotome	Present	Regionally restricted	3d right	Expression is present in a swatch of dermomyotome-derived cells extending from the top of the head to the tip of the tail on both sides of the embryo.
TS20: paraxial mesenchyme	Present	Regionally restricted	3d right	Expression is present in a swatch of dermomyotome-derived cells extending from the top of the head to the tip of the tail on both sides of the embryo.
TS20: tail dermomyotome	Present	Regionally restricted	3d right	Expression is present in a swatch of dermomyotome-derived cells extending from the top of the head to the tip of the tail on both sides of the embryo.
TS20: limb	Present	Regionally restricted	3d right

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Weak	Regionally restricted	4b	Expression is present in two narrow stripes.
TS23: vertebra	Weak		4b

Structure	Level	Pattern	Image	Note
TS23: hindbrain	Strong		4c right
TS23: midbrain	Strong		4c right	Regions of expression include the periaqueductal gray.
TS23: cerebellum	Present	Regionally restricted	4c right	Expression is present in a few patches.
TS23: inferior colliculus	Weak		4c right
TS23: pons	Present		4c right
TS23: embryo	Present	Regionally restricted	4c right	Expression is present under the skin, in dermomyotome-derived tissue.

Structure	Level	Pattern	Image	Note
TS23: midbrain	Strong		4d right
TS23: hindbrain	Strong	Regionally restricted	4d right	Expression is limited to a ventral patch of differentiated cells.
TS23: cerebellum	Present	Regionally restricted	4d right	Expression is present in a few patches.
TS23: inferior colliculus	Weak		4d right
TS23: embryo	Present	Regionally restricted	4d right	Expression is present under the skin, in dermomyotome-derived tissue.

Structure	Level	Pattern	Image	Note
TS25: cerebellum	Present		5a right
TS25: pons	Present	Regionally restricted	5a right	Expression extends from the junction of the cerebellum and pons rostrally into the midbrain.
TS25: midbrain	Present		5a right

Structure	Level	Pattern	Image	Note
TS25: cerebellum	Present		5b right
TS25: pons	Present	Regionally restricted	5b right	Expression extends from the junction of the cerebellum and pons rostrally into the midbrain.
TS25: hindbrain	Present		5b right

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Absent		6b right
TS28: periaqueductal grey	Present	Regionally restricted	6b right	Expression is present in the ventral, but not dorsal, periaqueductal gray; it extends forward from under the rostral portion of the cerebellum to the midbrain.
TS28: pons	Present		6b right	Areas of expression include the raphe linearis.
TS28: interpeduncular nucleus	Present		6b right

Structure	Level	Pattern	Image	Note
TS28: tectum	Absent		6c right	There is no expression in the colliculi.
TS28: substantia nigra	Present		6c right
TS28: pons	Present		6c right