Probe:	pFRK16
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		2A/B
TS15: future hindbrain	Present		2A/B
TS15: future midbrain	Present		2A/B
TS15: future spinal cord	Present		2A/B
TS15: dorsal root ganglion	Present		2A/B	Expressed in the differentiating neural crest cells.
TS15: dorsal aorta	Present		2A/B	Expressed in the migrating presympathetic neural crest cells.
TS15: midbrain-hindbrain junction	Present	Regionally restricted	2A/B , 2C/D	Expressed in a specific set of morphologically indistinguishable cells located at the fold.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present	Regionally restricted	2E/F	Expressed in a bilaterally symmetric profile along the lateral neuroepithelium of the myelencephalon.
TS15: trigeminal preganglion	Present		2E/F

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Strong	Regionally restricted	3A/B	Expressed in the lateral walls.
TS21: trigeminal nerve maxillary division	Strong		3A/B
TS21: trigeminal nerve ophthalmic division	Strong		3A/B
TS21: spinal cord	Present		3D/E , 3A/B
TS21: dorsal root ganglion	Present	Regionally restricted	3A/B , 3D/E	Expressed in a subset of neurons.
TS21: sciatic nerve	Present		3D/E , 3A/B	Expressed in the ventral ramus.
TS21: autonomic nervous system	Present	Regionally restricted	3A/B , 3D/E	Expressed in autonomic ganglion.

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Strong		4A/B
TS24: olfactory lobe	Strong		4A/B
TS24: spinal cord	Strong		4A/B
TS24: hindbrain ventricular layer	Strong	Regionally restricted	4A/B	Expressed in the ependymal layer of the fourth ventricle.
TS24: hindbrain	Strong	Regionally restricted	4A/B	Expressed in medullar auricle.
TS24: cranial nerve	Present		4A/B	Expressed in a complex pattern that outline innervation pathways.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus layer	Present	Regionally restricted	5A/B	Expressed in the pyramidal layer.
TS28: 3rd ventricle ependyma	Present		5A/B
TS28: lateral ventricle choroid plexus	Present		5A/B
TS28: lateral ventricle ependyma	Present		5A/B
TS28: mammillary body	Present		5A/B
TS28: medial habenular nucleus	Present		5A/B
TS28: fimbria hippocampus	Absent		5A/B
TS28: parafascicular nucleus	Absent		5A/B
TS28: reticular thalamic nucleus	Absent		5A/B

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		5C
TS28: vestibular nucleus	Strong		5C
TS28: cerebellar peduncle	Strong	Regionally restricted	5C	Expressed in the caudal peduncle.
TS28: trigeminal nerve	Strong	Regionally restricted	5C	Expressed in the trigeminal motor and spinal tracts in the brainstem.
TS28: cerebellum granule cell layer	Absent		5C

Structure	Level	Pattern	Image	Note
TS17: peripheral nerve trunk	Present			Expressed in the morphologically discernible neural crest-derived sympathetic chains.
TS17: autonomic nervous system	Present			Expressed in the plexuses.

Structure	Level	Pattern	Image	Note
TS28: corpus callosum	Absent
TS28: reticular thalamic nucleus	Absent