Gene Expression Data
Assay Details
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Assay
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Reference:
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J:9911
Davidson D, et al., A gene with sequence similarity to Drosophila engrailed is expressed during the development of the neural tube and vertebrae in the mouse. Development. 1988 Oct;104(2):305-16
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5469173
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Gene symbol:
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En1
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Gene name:
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engrailed 1
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Probe:
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p-lambda-4L7
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Assay notes:
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The authors did not specify which probe was used to obtain this data; it was was assumed that they used the probe that detects the expression of both family members.
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Results
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Specimen
not shown:
embryonic day 9.5
(more  )
Specimen
not shown:
(close  )
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Genetic Background:
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Swiss
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube |
Present |
Regionally restricted |
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Expression is present in the ventral part of the neural tube in the cervical and upper thoracic regions. It is most intense in a restricted, midlateral, region of the germinal zone. Using gene-specific probes, this is believed to be due to En1.
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| TS15: tail future spinal cord |
Absent |
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There is no expression in the caudal part of the tail.
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| TS15: trunk dermomyotome |
Moderate |
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Based on experiments using gene-specific probes, this expression believed to be due to En1.
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| TS15: tail paraxial mesenchyme |
Absent |
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Expression levels are barely above background.
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| TS15: head mesenchyme |
Present |
Regionally restricted |
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Expression is present in the facial mesenchyme anterior to the forebrain, above the developing snout; there is no expression in the lateral parts of face. Based on experiments using gene-specific probes, this expression believed to be due to En1.
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Specimen
not shown:
embryonic day 10.5
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
Swiss
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Present |
Regionally restricted |
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Expression is present in the ventral part of the neural tube in the cervical and upper thoracic regions. It is most intense in a restricted, midlateral, region of the germinal zone. Using gene-specific probes, this is believed to be due to En1.
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| TS17: tail future spinal cord |
Absent |
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There is no expression in the caudal part of the tail.
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| TS17: trunk dermomyotome |
Present |
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Based on experiments using gene-specific probes, this expression believed to be due to En1.
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Analysis Tools
