Probe:	p-lambda-4U6
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present
TS20: midbrain-hindbrain junction	Present
TS20: hindbrain floor plate	Present			Expression is present in the floor of the hindbrain.
TS20: spinal cord	Present			Expression is present in the neural tube.
TS20: dermomyotome	Present
TS20: sclerotome	Present			Expression is present in the sclerotome of developing centra.
TS20: vertebral cartilage condensation	Present			Expression is present in the costal processes and neural arches.
TS20: head mesenchyme	Present	Regionally restricted		Expression is present in the facial mesenchyme.
TS20: limb epithelium	Present			Expression is present in limb bud ectoderm.
TS20: body wall	Absent			There is no expression in thoracic body wall.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	2C/D	Expression is present in the ventral part of the neural tube from the junction with the hindbrain to at least the level of the 30th segment posterior to the atlas.
TS20: head mesenchyme	Present	Regionally restricted	2C/D	Expression is present in the facial mesenchyme anterior to the forebrain, above the developing snout; there is no expression in the lateral parts of face.
TS20: vibrissa	Absent		2C/D
TS20: dermomyotome	Present		2C/D	There is a complex pattern of expression.
TS20: sclerotome	Present		2C/D	There is a complex pattern of expression.
TS20: hindbrain	Present		2C/D
TS20: midbrain	Present		2C/D
TS20: midbrain-hindbrain junction	Present		2C/D
TS20: vertebral cartilage condensation	Present		2C/D

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	3E	Expression is present in discrete domains in the germinal and intermediate zones.
TS20: midbrain	Present	Regionally restricted	3E	Expression is present in discrete domains in the germinal and intermediate zones.
TS20: midbrain-hindbrain junction	Present	Regionally restricted	3E	Expression is present in the roof.
TS20: hindbrain floor plate	Present		3E	Expression is present in the germinal and intermediate zones in the anterior floor of the hindbrain.
TS20: midbrain floor plate	Strong		3E
TS20: pons	Strong		3E

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	4C/D	Expression is present in the ventral part of the neural tube from the junction with the hindbrain to at least the level of the 30th segment posterior to the atlas.

Structure	Level	Pattern	Image	Note
TS20: thoracic vertebral cartilage condensation	Present		9A/B	Expression is present in segmental blocks of mesenchyme in the positions expected of the developing costal process.