Gene Expression Data
Assay Details
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Assay
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Reference:
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J:39412
Dechesne CJ, et al., Rab3A immunolocalization in the mammalian vestibular end-organs during development and comparison with synaptophysin expression. Brain Res Dev Brain Res. 1997 Mar 17;99(1):103-11
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5468985
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Gene symbol:
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Rab3a
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Gene name:
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RAB3A, member RAS oncogene family
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Antibody:
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3Ap-3
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Detection system:
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Secondary antibody coupled to Texas Red
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Results
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Specimen
1a1:
embryonic day 13.5
(more  )
Specimen
1a1:
(close  )
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Genetic Background:
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involves: C57 * CBA
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
not shown:
embryonic day 14.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: C57 * CBA
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1b1:
embryonic day 15.5
(more )
Specimen
1b1:
(close )
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Genetic Background:
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involves: C57 * CBA
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: vestibular component epithelium |
Present |
Regionally restricted |
1b1
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Expression was detected in the afferent neurites within the sensory epithelia and around the sensory cells. Strongly stained patches were present at the base of the sensory cells.
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| TS23: utricle |
Present |
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1b1
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| TS23: semicircular canal |
Present |
Regionally restricted |
1b1
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Expression was detected in the central zones of the cristae.
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Specimen
1c1:
embryonic day 16.5
(more )
Specimen
1c1:
(close )
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Genetic Background:
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involves: C57 * CBA
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: utricle |
Present |
Punctate |
1c1
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Expression was detected in afferent endings.
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| TS24: semicircular canal |
Present |
Regionally restricted |
1c1
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Expression was dected in the cristae. Hair cells were not stained.
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Specimen
1d1:
embryonic day 18.5
(more )
Specimen
1d1:
(close )
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Genetic Background:
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involves: C57 * CBA
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: utricle |
Present |
Regionally restricted |
1d1
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The pattern of staining was identical but denser than that at E16.5. Labeled fibers formed a dense plexus at the base of the sensory cells and long punctuate neurites along them. Rare hair cells were faintly stained.
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| TS26: crista ampullaris |
Present |
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1d1
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Specimen
2a:
postnatal newborn
(more )
Specimen
2a:
(close )
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Genetic Background:
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involves: C57 * CBA
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: vestibular epithelium |
Strong |
Regionally restricted |
2a
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Expression was observed in numerous afferent neurites in the sensory epithelia.
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Specimen
2b1:
postnatal newborn
(more )
Specimen
2b1:
(close )
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Genetic Background:
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involves: C57 * CBA
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: vestibular epithelium |
Present |
Punctate |
2b1
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Expression formed patches at the base of the hair cells and in the thin neurites growing almost to the apex of the sensory cells. Some sensory cells were strongly labeled.
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Specimen
3a1:
postnatal day 9
(more )
Specimen
3a1:
(close )
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Genetic Background:
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involves: C57 * CBA
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Age:
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postnatal day 9
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: vestibular epithelium |
Present |
Regionally restricted |
3a1
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Expression was detected in numerous afferent calyces and patches localized at the base of the sensory cells. Staining was intense in the upper part of the calyces. Moderate to intense staining was noted in the hair cell cytoplasms.
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Specimen
3e1:
postnatal day 20
(more )
Specimen
3e1:
(close )
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Genetic Background:
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involves: C57 * CBA
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Age:
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postnatal day 20
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: vestibular epithelium |
Present |
Regionally restricted |
3e1
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Expression was detected in the nerve calyces and endings at the base of the sensory cells. Staining was noted in the hair cell cytoplasms.
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