Probe:	IMAGE clone 536389
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: mesonephric tubule	Present		2A

Structure	Level	Pattern	Image	Note
TS17: mesonephric tubule	Present		2B
TS17: nephric duct	Absent		2B

Structure	Level	Pattern	Image	Note
TS20: comma-shaped body	Strong		2C
TS20: metanephros	Strong	Regionally restricted	2C	Expression is surrounding the ureter stalk.
TS20: epithelial layer of ureter	Absent		2C

Structure	Level	Pattern	Image	Note
TS20: comma-shaped body	Strong		2D
TS20: metanephros	Strong	Regionally restricted	2D	Expression is surrounding the ureter stalk.
TS20: epithelial layer of ureter	Absent		2D

Structure	Level	Pattern	Image	Note
TS22: s-shaped body	Present		2E

Structure	Level	Pattern	Image	Note
TS23: comma-shaped body	Present		2F
TS23: s-shaped body	Present		2F
TS23: maturing glomerular tuft	Present		2F	Expression is confined to the glomerular precursors of the outer cortical region but missing in the inner part of the developing kidney where tubuli and mature glomeruli are located.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Strong	Regionally restricted	4I	Stronger staining in odd-numbered rhombomeres.
TS12: neural tube	Present	Regionally restricted	4I	Expression is ventral.
TS12: future prosencephalon	Present		4I

Structure	Level	Pattern	Image	Note
TS13: rhombomere 1	Strong		4J
TS13: rhombomere 3	Strong		4J
TS13: rhombomere 5	Strong		4J
TS13: neural tube	Present		4J
TS13: rhombomere 2	Present		4J
TS13: rhombomere 4	Present		4J

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present		4K
TS15: mesonephros	Present		4K
TS15: neural tube	Present		4K
TS15: optic cup	Present		4K

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present		4L
TS15: mesonephros	Present		4L
TS15: neural tube	Present		4L
TS15: optic cup	Present		4L

Structure	Level	Pattern	Image	Note
TS17: 2nd branchial groove ectoderm	Present		4M
TS17: 3rd branchial groove epithelium	Present		4M
TS17: eye	Present		4M
TS17: neural tube	Present	Regionally restricted	4M	Excpression is in the ventral neural tube.
TS17: hindbrain	Present	Regionally restricted	4M	Expression is in specific rhombomeres and prosomeres.

Structure	Level	Pattern	Image	Note
TS17: brain	Present		4N	Expression in the basal and alar plate.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	4O	Expression is in longitudinal stripes.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	4P	Expression is in a short stretch in the caudal part of the rhombencephalic basal plate.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	4Q	Excpression is in the ventral neural tube.
TS17: neural tube floor plate	Absent		4Q

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	4R	Expression is in the ventral ependymal layer.

Structure	Level	Pattern	Image	Note
TS14: optic vesicle	Present			Optic placode.

Structure	Level	Pattern	Image	Note
TS17: lens pit	Present		5E
TS17: optic cup inner layer	Present		5E

Structure	Level	Pattern	Image	Note
TS20: neural retinal epithelium	Present	Regionally restricted	5F	Expression is in the outer neuroblast layer.

Structure	Level	Pattern	Image	Note
TS22: lens anterior epithelium	Strong		5G
TS22: neural retinal epithelium	Present	Regionally restricted	5G	Expression is in the outer neuroblast layer.

Structure	Level	Pattern	Image	Note
TS23: neural retina	Present		5H	Expression is in the outer neuroblast layer.
TS23: lens anterior epithelium	Weak		5H

Structure	Level	Pattern	Image	Note
TS20: lower leg mesenchyme	Present	Regionally restricted		Expression is observed lateral to the separating tibia and fibula.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	6A	Expression is in the shoulder and at the distal end of the cartilaginous condensation.

Structure	Level	Pattern	Image	Note
TS20: foot	Present	Regionally restricted	6B	Expression is extending along the digital rays.
TS20: hand	Present	Regionally restricted	6B	Expression is extending along the digital rays.

Structure	Level	Pattern	Image	Note
TS21: foot joint	Strong		6C
TS21: hand joint	Strong		6C

Structure	Level	Pattern	Image	Note
TS22: foot	Strong	Regionally restricted	6D	Expression is at the interphalangeal joints.

Structure	Level	Pattern	Image	Note
TS23: hindlimb	Present	Regionally restricted	6E	Expression is in joints and it is weaker in the more proximal joints.

Structure	Level	Pattern	Image	Note
TS22: sternum	Present	Regionally restricted	6F	Expression is between the sternal bands and where the ribs contact the sternum.

Structure	Level	Pattern	Image	Note
TS23: hindlimb	Present	Regionally restricted	6G	Expression is in joints and it is weaker in the more proximal joints.

Structure	Level	Pattern	Image	Note
TS23: head	Present	Regionally restricted	7B	Expression is surrounding cartilaginous and epithelial structures in the skull.
TS23: face mesenchyme	Present	Regionally restricted	7B	Surrounding the whisker follicles.

Structure	Level	Pattern	Image	Note
TS23: nose	Present	Regionally restricted	7E	Expression is in surrounding epithelium and cartilage.

Structure	Level	Pattern	Image	Note
TS24: molar enamel organ	Present		7H

Structure	Level	Pattern	Image	Note
TS19: mandibular process mesenchyme	Present
TS19: maxillary process mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS19: esophagus	Present		8B

Structure	Level	Pattern	Image	Note
TS23: ureter	Present		8C

Structure	Level	Pattern	Image	Note
TS22: dorsal aorta	Present		8D
TS22: ductus arteriosus	Present		8D