Probe:	probe 3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS8: embryo	Absent

Structure	Level	Pattern	Image	Note
TS9: embryo	Present	Regionally restricted	4B/C	Expression is in a semicircular band that stretches partway around the circumference of the embryo and is about 5-10 cells wide along the proximal- distal axis. In epiblast cells on one side only.
TS9: embryo ectoderm	Present	Regionally restricted	4B/C	Expression is in proximal embryonic ectoderm on both sides of the proamniotic cavity.
TS9: extraembryonic visceral endoderm	Present	Regionally restricted	4B/C	Expression is in a cluster of endodermal cells adjacent and distal to the expressing embryonic ectoderm.
TS9: extraembryonic ectoderm	Absent		4B/C
TS9: parietal endoderm	Absent		4B/C

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present		4E/F
TS10: embryo ectoderm	Present	Regionally restricted	4E/F	Expression is in lateral ectodermal cells near the embryonic-extraembryonic junction.
TS10: embryo mesoderm	Present	Regionally restricted	4E/F	Anterolateral.
TS10: extraembryonic visceral endoderm	Present	Regionally restricted	4E/F	In cells that lie distal to the embryonic mesoderm layer.

Structure	Level	Pattern	Image	Note
TS10: extraembryonic mesoderm	Present

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Strong	Graded	5A/B	Highest levels are in the proximal region.
TS10: extraembryonic ectoderm	Absent		5A/B
TS10: extraembryonic endoderm	Absent		5A/B

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Strong	Graded	5C/D	Highest levels are in the proximal region.
TS10: embryo mesoderm	Present	Graded	5C/D	Higher levels in posterior mesoderm cells.
TS10: embryo ectoderm	Strong	Graded	5C/D	Higher levels in posterior cells, the anterolateral limit is close to the streak in the ectoderm than in the mesoderm.

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Strong	Graded	5E/F	Highest levels are in the proximal region.
TS10: embryo mesoderm	Present	Graded	5E/F	Higher levels in posterior mesoderm cells.
TS10: embryo ectoderm	Strong	Graded	5E/F	Higher levels in posterior cells, the anterolateral limit is close to the streak in the ectoderm than in the mesoderm.
TS10: embryo endoderm	Present	Regionally restricted	5E/F	Expression is where the subjacent mesoderm and/or ectoderm is positive.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present	Graded	6B/C	Highest levels are in the proximal region.
TS11: embryo	Present	Regionally restricted	6B/C	Expression is in a region that contains the distal tip of the egg cylinder that contains a portion of the head process.

Structure	Level	Pattern	Image	Note
TS11: allantois	Present	Regionally restricted		Expression is at the base, absent in proximal parts.
TS11: extraembryonic mesoderm	Absent			Absent in the extraembryonic mesoderm that lines the exocoelom.

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present	Graded	7A/B
TS12: embryo ectoderm	Present	Regionally restricted	7A/B	Expression is in mesoderm and ectoderm lateral to the primitive streak.
TS12: embryo mesenchyme	Present	Regionally restricted	7A/B	Expression is in mesoderm and ectoderm lateral to the primitive streak.
TS12: allantois	Present	Regionally restricted	7A/B	Expression is at the base.

Structure	Level	Pattern	Image	Note
TS12: extraembryonic mesenchyme	Absent			Absent in proximal mesoderm.

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present	Graded	7C/D
TS12: embryo ectoderm	Present	Regionally restricted	7C/D	Expression is in mesoderm and ectoderm lateral to the primitive streak.
TS12: embryo mesenchyme	Present	Regionally restricted	7C/D	Expression is in mesoderm and ectoderm lateral to the primitive streak.

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present		8B/C
TS13: embryo ectoderm	Present	Regionally restricted	8B/C	Expression is in mesoderm and ectoderm lateral to the primitive streak.
TS13: embryo mesenchyme	Present	Regionally restricted	8B/C	Expression is in mesoderm and ectoderm lateral to the primitive streak.
TS13: allantois	Present	Regionally restricted	8B/C	Expression is at the base.

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present	Graded	8D/E

Structure	Level	Pattern	Image	Note
TS17: hindgut	Present	Regionally restricted	9A/B	Posterior expression.
TS17: tail mesenchyme	Present		9A/B

Structure	Level	Pattern	Image	Note
TS14: tail bud	Present	Regionally restricted		Expression is in the tail bud mesenchyme.
TS14: hindgut diverticulum	Present	Regionally restricted		Expression is in the posterior part.

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present